The Experimental Research Of Effects Of ShenQi Compound Recipe On Oxidative Stress And The Key Enzyme In GK Rats With T2DM Macroangiopathy

Objective:To research the effect of ShenQi prescription, which has the function of tonifying Qi and Yin and promoting blood circulation, on the oxidative stress and the expression of key enzymes in GK rats (Goto-Kakizaki wistar rats) with T2DM Macroangiopathy, and to find out the possible pathway.Methods:Departed55GK male rats (RBS≥11.lmmol·L-1) as GK group (14rats, drenched normal saline5ml·kg-1·d-1), model group (13rats, drenched normal saline5ml·kg-1·d-1), atorvastatin group (14rats, drenched atorvastatin calcium suspension1.6mg·kg-1·d-1), ShenQi prescription group (14rats, drenched ShenQi compound recipe suspl1.44g·kg-1·d-1) and14Wistar rats act(RBS≤6.7mmol·L-1) as the normal group (drenched normal saline5ml·kg-1·d-1). GK rats were fed with high fat diet, while Wistar rats with general diet. The Nco-nitro-L-arginine methyl ester (L-NAME)0.1mg.ml-1.d-1was added into the drinking water for model, atorvastatin and ShenQi group to build the diabetes mellitus macroangiopathy model. Treating while setting up model. The experiment required35days. In the experiment period, general status were observed as well as the amount of water drinking, food intake, blood dlucose and body weight. At the end, detect the SOD by Xanthine oxidase, detect the GSH-Px and T-AOC by ABC-ELISA, detect the MDA by TBA, detect the erythrocyte AR by Fluorescence Method, estimated the mRNA expression of p22phox and p47phox in thoracic aorta vessel wall by real-time PCR, detect abdominal aorta vessel wall by HE Staining.Results:ShenQi prescription can improve GK rats" common state. The ShenQi group had significantly higher SOD, GSH-Px and TAOC activity in blood serum compared with model group(P<0.01or P<0.05). ShenQi prescription can decrease the activity of erythrocyte AR(P<0.01), restrain the mRNA expression of p22phox mRNA and p47phox mRNA in thoracic aorta vessel wall (P<0.01).Conclusion:Improved the activities of antioxidant enzymes (including SOD and GSH-Px), increase the Total antioxidant capacity (T-AOC)decrease the activity of erythrocyte AR, significantly downregulated the mRNA expression of p22phox and p22phox in thoracic aorta vessel wall maybe one of the possible curing mechanisms that inhibit the oxidative stress and prevented T2DM Macroangiopathy by ShenQi prescription.