Synovial Mesenchymal Stem Cells To Chondrogenesis Induced By Chondrocytes On The Three-dimensional Scaffold In Vivo

Objective:Synovial mesenchymal stem cells have the ability of multipotential differentiation, which are expected to be seed cells for the treatment of cartilage defects in cartilage tissue engineering.Compared to other sources of MSCs has a higher chondrogenic capacity, To investigate the differential ability of chondrogenesis by co-culture synovial mesenchymal stem cells and chondrocytes from SD rats in the chitosan/I collagen composite scaffolds after being transplanted into subcutaneous layer of SD rats. Methods:(1)The synovial mesenchymal stem cells and chondrocytes harvested from synovial membrane and articular cartilage of SD rat were obtained by enzyme digestion and cultured respectively,(2) the passage 3 synovial mesenchymal stem cells and passage 2 chondrocytes were divided into four groups, respectively, group A(chondrocytes), group B(synovial mesenchymal stem cells), group C(synovial mesenchymal stem cells:chondrocytes=1:2) and group D(scaffold material without cells) were cultured on chitosan/I collagen composite scaffolds and transplanted into subcutaneous layer of SD rats and observed morphology,chemically stained after 4 weeks and 8 weeks. Results:After 4 weeks and 8 weeks scaffold material can be maintained disc. The immunohistochemical staining of collagen II and the toluidine blue of aggrecan were significantly positive from group A and group C. Conclusion:The results showed that cells-scaffold compound co-cultured in vivo can form cartilage-like tissue.