Separation And Detection Circulating Tumor Cells Based On Magnetic Separation Technology And Spectrum Technology

Nanotechnology is based on modern technology combining modern science with modern technology. This technology is an interdisciplinary to research the properties and applications of material whose partical size is in the range of 1nm to 100 nm. As the special chemistry and physics property, nanomaterial is used in the physical testing and biological applications and so on. Nanotechnology has high sensitivity and efficiency on the testing of substances. Circulating tumor cell(CTC) is a small number of tumor cells which exists in peripheral blood. This tumor can transfer to other organs through blood. The transfer of tumor cells is the main reason to make the death of patients. In the early phase, the diagnosis of circulating tumor cell conduces to identify benign and malignant tumors and transfer risk prediction and assessment. So the early diagnosis and treatment to CTC have important implication to cancer patients. Immunomagnetic separation technology is an immunological technique combining high specificity of immunology with magnetic responsiveness of magnetic beads. This paper is devoted to detect and separate the circulating tumor cells through the magnetic separation technology. The Immunomagnetic and the Raman nanoparticle in this paper have small partical size, uniform dispersion and high specificity. This paper structure the good performance method to capture and detect circulating tumor cells through combining Immunomagnetic separation technology with indirect immunofluorescent technology or surface enhanced Raman scattering technology. The main researches are as follows.1. Based on the development and application of magnetic separation technology in the tumor cell sorting, the circulating tumor cells can connect with the specific proteins on the Immunomagnetic’s surface. The aim cells can be separated from the mixed cells in the magnetic field. The separated cells can connect with the specific antibody with PE fluorophore. This method can quantitative detect tumor cells through the observation of inversion fluorescence microscope. In this experiment, the aim cell is lung cancer A549 cell, the control cell is MRC-5 cell. The lower limit of detection is three cells per milliliter. This experiment can separate and detect circulating tumor cells through the Immunomagnetic separation technology and indirect immunofluorescent technology.2. Based on the development and application of magnetic separation technology in the tumor cell sorting, the aim cells can be separated from the mixed cells in the magnetic field. The synthetic Raman nanoparticle AuNPs-MBA-BSA-FA can specific connect with the aim cells. These Raman nanoparticles have small partical size, uniform dispersion, high specificity and strong Raman signal. In this experiment, the aim cell is cervical cancer Hela cell, the control cell is MRC-5 cell. The lower limit of detection is six cells per milliliter. This experiment can separate and detect circulating tumor cells through the Immunomagnetic separation technology and surface enhanced Raman scattering technology.