Studies On The Chemical Constituents And Bioactivities Of Lindera Nacusua (D.Don) Merr

In this dissertation, the chemical constituents of Lindera nacusua (D. Don) Merr. were investaged. The ethanol extract of L. nacusua was obtained by diacolation method, then the ethanol extract was suspended in water (50℃), and was extracted with petroleum ether, ethyl acetate and n-butanol, respectively, each for three times. The chemical constituents of ethyl acetate were studied by a variety of separation methods. Finally, eighteen compounds have been isolated from the ethyl acetate part of the roots of L.nacusua. And the structures of the fourteen compounds had been identified by HR-ESI-MS,1H NMR,13C NMR,2D NMR, UV, IR, and combined with TLC method, two steroids (compounds 1,2), anthraquinones (compounds 3,6), y-butanolides (compounds 4,5), phenolic glycosides (compounds 7-12), phenolic acid amides (compounds 17-18). They were identified as:β-Sitosterol (1), β-Daucosterol (2), Demethylmacrosporine Ⅰ (3), (-)-Litsenolide D2 (4), (+)-(2Z,3R, 4S)-2-(Dodec-11-ynylidene)-3-hydroxy-4-methylbutanolide (5), Emodin-6-O-β-D-glucopy-ranoside (6), 1-O-3-Hydroxyphenyl-5-methoxyphenol-(6’-O-vanilloyl)-β-D-glucopyranoside (7),6-Hydroxy-4-methoxyphenol 1-O-β-D-(6’-O-syringoyl) glucopyranoside (8),4-Hydrox-y-6-methoxyphenol 1-O-β-D-(6’-O-syringoyl) glucopyranoside (9),6’-O-Vanilloylisotachio-side (10), 1-O-3-Hydroxyphenyl-4,5-dimethoxy-(6’-O-3",5"-dimethoxygalloyl)-β-D-gluc-opyranoside (11), 1-O-3-Hydroxyphenyl-4,5-dimethoxy-(6’-O-vanilloyl)-β-D-glucopyrano-side (12), N-Trans-feruloyltyramine (17), N-Cis-feruloyltyramine (18).Compound 7 was a new structure, compound 4 was a new natural product, compounds 3,5,6,8-12 were isolated for the first time from the Lauraceae family, and all the compounds were isolated from this plant firstly.In addition Compounds 3-5 were biological activity of antitumor by MTT method in vitro., compound 3 showed growth inhibition on breast cancer MDA-MB-231 cells, the mechanism may be related to cut the expression of FAS, and compound 4 exhibited effective growth inhibition on colon cancer HT-29 and SW-620 cells, compound 5 significant growth inhibition on breast cancer MDA-MB-231 cells, the IC50 values were 39,34,26,11μM, respectively.