Application Of DNA Image Cytometry In The Diagnose Of Lung Cancer

Objective: To investigate the value of DNA image cytometry in the diagnose of lung cancer by comparing with the Liquid-based cytology results, looking for reliable method in early diagnosis of lung cancer.Materials and Methods: Using DNA image cytometry and Liquid-based cytology of 570 lung cancer group specimens(including 246 sputum specimens, 226 bronchoalveolar lavage fluid specimens, 98 bronchial brushing specimens),and 465 lung cancer group specimens(including 213 sputum specimens, 205 bronchoalveolar lavage fluid specimens, 47 bronchial brushing specimens).Results: The sensitivity of DNA-ICM of BALF and sputum cytology were 71.2% and 41.9%, while LBC were 49.1% and 22.4%. The sensitivity of DNA-ICM of BALF and sputum cytology were higher than that of method of LBC(P<0.01). There were 117 patients who underwent BALF and bronchial brushing cytology, The sensitivity and specificity of DNA-ICM of BALF were 77.5% and 100%, The sensitivity and specificity of LBC of bronchial brushing were 58.8% and 100%. The sensitivity of DNA-ICM of BALF was higher than that of bronchial brushing cytology(P<0.05). Of the 246 cases which had histopathological results, the sensitivity of adenocarcinoma was 59.8%, the sensitivity of squamous carcinoma was 77.4%, the sensitivity of squamous carcinoma was higher that of adenocarcinoma(P<0.01).Conclusions: DNA-ICM has great application value in the diagnose of lung cancer, both in the sputum and bronchoalveolar lavage fluid specimens, the sensitivity of DNA-ICM of BALF and sputum cytology were higher than that of method of LBC, in a certain extent, can reduce the missed diagnosis; and can increase the positive rate with Liquid-based cytology; BALF is more preponderant than bronchial brushing cytology by DNA-ICM in the diagnose of lung cancer which can be applied in clinical.