The Identification And Quality Standard Of Rumex Madaio Makino R. Daiwoo Makino

Objective: Using traditional identification methods, molecular biology identification method to research source identification of rumex medicinal materials, at the same time, establish the research on medicinal materials and yinpian of Rumex madaio Makino R.quality standards.Methods:Retrieving all previous dynasties materia medica, standard and processing; 55samples(of which used to identify research were 35 samples, 20 samples were used in the study of quality standard) were got in field study; Use a variety of methods including the original plant material object identification, character identification, microscopic identification and the DNA molecular identification rhubarb for qualitative identification of Rumex madaio Makino R.; The First time to adopt the method of specific primer PCR detection rumex belong to many kinds of plants and falsify mountain rhubarb.Clear and definite species contained in Shanxi local quality standard based on Refe-rencing other Rumex madaio Makino R. quality standards, included clinical medication habits; Using TLC to monitor conduct authenticity and qualitative identification, inspection; Using HPLC to nepodin-8-O-β-D-glucopyranoside, emodin, chrysophanol, physcion content determination; analysis of drug impurity guidelines to test the moisture content, ash content, etc. On The Chinese pharmacopoeia 2015 edition of four appendices limited test.Results: 1.Through the combination of traditional and DNA barcode identification appraisal, the results showed that 35 rumex samples mark 2, 8, 11, 13, 14, 15, 18, 19, 23, 24, 25, 28, 29, 30, 32, 33 were Rumex patientia L.; No. 3, 20, 21 were Rumex crispus L.; No.5, 6 were Rumex maritimus L.; No.1, 9, 12, 31 were Rumex japonicas Houtt.; No. 4, 7 can be determined rumex, unable to determine the kinds of species; No. 22, 26 were Rumex dentatus L.; No.10 was Rumex nepalensis Spreng.; No.16, 17 were Rumex hadroocarpusRech.; No. 34, 35 were falsify species; Extractionn DNA of No. 27 was failure because of dated; Based on the model of various plants psb A-trn H sequence, designed a pair of specific primers by polymerase chain reaction can quickly detect sorrel belong to many kinds of plants and falsify.Comprehensive the above results will place in Shanxi Province are determined standard middle-earth rhubarb base source quasi contains Rumex patientia L., Rumex crispus L. and Rumex dentatus L.2. Establish TLC identification and inspection method.By using thin layer chromate-graphy analysis found that plants of the rumex that emodin, chrysophanol, physcion composition;Also contains nepodin-8-O-β-D-glucopyranoside,which does not be contained in the soil rhubarb glycosides, can be detected to differentiate false species; For this study respectively established [identify], [check].3. Establishing HPLC content determination method.This study used in Littleton Hypersil BDS C18(250 mm *4.6 mm, 5 microns) chromatographic column: 0.1% phos-phorric acid with methanol water gradient elution sequence of 0 to 14 min(B % of 60%-39%), 14 to 15min(B % of 39%- 16%), 15-38min(B % 16%); Flow rate: 1.0 ml·min-1; Detection wavelength of 225 nm, 225 nm can be the content determination method of simultaneous determination of acid plant acid mode glycosides, emodin, chrysophanol, physcion.Conclusion:1. The plant traits associated with bar code identification, the DNA mole-cule can better solve acid complex plant classification and appraisal difficult problem.2. The content of four kinds of chemicals Rumex japonicus Houtt. higher than other several rumex materials.The findings could provide experimental basis for soil classifyca-tion research.3. Rhaponiticin did not exit in rumex, but in false species, Rhaponiticin could be as rumex inspection items, can achieve authenticity identification; In this paper, the thin layer identification method and the map spots were clear, good separating degree; Four major component content determination method is simple, good repeatability, stability, sample recovery rate meets the requirements.The established qualit y standards can be used for rumex quality control of medicinal herbs or slices.