| Objective:To optimize fermentation conditions on the basis of Inonotus obliquus as fermentation substrate and polysaccharides, β-glucan content as a reference standard, and investigate the hypoglycemic effect of Inonotus obliquus optimized fermentation product on type 2 diabetic rats.Method:(1)To design a single factor experiment on the basis of the single factor effect of pH, temperature, incubation time on polysaccharide,β-glucan content of fermented products, and obtain optimal fermentation conditions on the basis of (β-glucan content as the standard, and measure the polysaccharide,β-glucan content of Inonotus obliquus optimized fermented product. Measure polysaccharide absorbance of prepared fermented Inonotus obliquus polysaccharide tested products that distilled water as blank at a wavelength of 492nm. Measure β-glucan absorbance of prepared fermented Inonotus obliquus P-glucan polysaccharide tested products that Congo red solution as blank at a wavelength of 555nm. (2) 110 healthy male SD rats were randomly divided into 2 groups:normal control group and diabetic group. Model group is establish to food-borne type 2 diabetic rat model by combining high fat diet with streptozotocin injection. The successful rats were randomly divided into six groups on the basis of blood glucose values:model group (distilled water 1ml/kg), unfermented Inonotus obliquus group (1000mg/kg,2000mg/kg), fermentation Inonotus obliquus group (1000mg/kg,2000mg/kg), a positive control group (metformin 90mg/kg). After 8 weeks, blood was drawn from the heart of all fasted 12h rats, and serum is separated and is measured content of serum free fatty acids and glucose. Record food intake of every group rats per day and weight change weekly during the experiment.Results:1) Obtain the best fermented conditions of Inonotus obliquus:Inonotus polysaccharide and P-glucan content of Inonotus obliquus culture can reach The highest peak at pH 5, culture temperature 50 ℃, culture time 7h.2) Compared with unfermented Inonotus obliquus, polysaccharide,|3-glucan levels of fermented Inonotus obliquus were significantly increased P<0.01).3) Compared with the normal group, after high fat diet for 4 weeks, the group of diabetic rats serum free fatty acid content increased (P<0.05).4) Compared with the normal group, the fasting blood glucose level of model group rats injected streptozotocin (30mg/kg)after 72h increased significantly (P<0.01).5) Compared with the model group, rats weight administering small dose of unfermented Inonotus obliquus treatment in 3th,4th,6th,7A weeks increased (P <0.05), In the 2th,5th,8th week have significant difference (P<0.01); rats weight administering large dose of unfermented Inonotus obliquus treatment in 1th,4th,6th,7th week increased (P<0.05), in the 2th,3th,5th,8th week have significant difference (P<0.01); rats weight administering small dose of fermented Inonotus obliquus treatment for 3,4 weeks increased (P<0.05), in the 2th,5th,6th,7th,8th week have significant difference (P<0.01); rats weight administering large dose of fermented Inonotus obliquus treatment for 1 week increased (P<0.05), in the 2 to 8th week is significant difference (P<0.01).6) Compared with the model group, the food intake of the unfermented Inonotus obliquus small dose group that feeding high-fat diet for 3 weeks increased (P<0.01), and has significant difference (P<0.05). the food intake of the unfermented Inonotus obliquus large dose group that feeding high-fat diet for 4 weeks increased (P<0.01), and has significant difference in 5th week(P<0.05). the food intake of the fermented Inonotus obliquus small dose group that feeding high-fat diet for 6,7,8 weeks increased(P<0.05), and has significant difference in 4th,5th week (P<0.01). the food intake of the fermented Inonotus obliquus large dose group that feeding high-fat diet in 1th,2th,5th week increased, and has significant difference in 4th,6th,7th,8th week(P<0.05). Compared with the unfermented Inonotus obliquus low dose group, food intake of the unfermented Inonotus obliquus high dose group increased in 1th,7th,8th week (P<0.05). Food intake of the fermented Inonotus obliquus small dose increased in 3th,4th,5th,6th weeks and has significant difference (P<0.01). Compared with the unfermented Inonotus obliquus high-dose group, food intake of the fermented Inonotus obliquus high dose group increased in 4th,6th,8th week (P<0.05), and has significant difference in the 7th week of (P<0.01).7) Compared with the model group, blood glucose level decreased significantly (P<0.01) in rats administrated fermented Inonotus obliquus high dose for 8 weeks; compared with the unfermented high-dose group, the fermented Inonotus obliquus high-dose group and the positive control group have significantly hypoglycemic effect (P<0.05).8) Compared with the model group, serum free fatty acid content in rats administrated fermented Inonotus obliquus large doses and low-dose group was significantly reduced (P<0.01); compared with the unfermented Inonotus obliquus, the fermented Inonotus obliquus have significantly adjustment effect on free fatty acids of type 2 diabetic rats (P<0.05). |
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