| Purpose:As non-steroidal anti-inflammatory drug utilization rate increase, the clinical correlation between stomach trouble also gradually increasing. In this paper, which looks flavored having spread of nonsteroidal anti-inflammatory drugs (NSAIDs) correlation between gastric ulcer rat gastric mucosa P-P38MAPK, TNF-a, PGE2, IL-1 expression and the effect of analysis flavored having NSAIDs correlation mechanism of action of gastric ulcer treatment.Method:Choose the weight of 200-240 g 46 only clean SD rats, male and female half and half. Randomly divided into 4 groups, group A for blank control group, group B for the model control group, group C for rabeprazole azole groups. Group D for flavored having group. Fasting,48 h, free drinking water. After 48 h B, C, D group lavage solution indomethacin 2.5 mg/kg,1/D,14 D. After the success of the building, B group was given 0.9% sodium chloride, give rabeprazole group C, D group was given Flavored Wu Qi Powder. Lavage noon 1 times a day, according to the ray Bella azole 2 mg/kg, having 2 g/kg, calculate the dosage. After 28 days fasting water for 24 h. According to the requirements of the animal experiment, human and rat drug dose conversion to conversion factor calculation method for flavored having scattered rats dose (notoginseng powder 0.04 g/kg and white powder 0.04 g/kg fructus schisandrae 0.04 g/kg nine incense insect powder 0.02 g/kg calcined cuttlefish bone powder 0.04 g/kg).29 days in the rat abdomen anesthesia backup skin, cut along the belly line, blood collection needle puncture abdominal aortic blood, isolated gastric tissue. Using immunohistochemical method to detect flavored having spread the expressionof TNF-a rat gastric mucosa, the influence of using Western blot test P-P38MAPK, cox-2 protein activity. With ELISA detection of PGE2, the expression of IL-1.Results:(1) general state and behavior:rabeprazole group, flavored scattered groups of rats having state of mental activity, fur color, diet, weight, etc have improved significantly in the model group, and rabeprazole group, flavored having general status of scattered group is superior to other groups.(2) the pathological morphological observation:blank group, gastric mucosa structure integrity, no congestion, edema, and so on and so forth, columnar epithelium is a single layer, neat rows, almost no defect, fall off, no inflammatory cell infiltration, lamina propria gland shape rules; Lesion model group:gastric mucosa was thinned, epithelial cell falls off, visible defects, lamina propria plenty of inflammatory cells infiltration, partly visible, with lymphoid follicular formation, disordered arrangement of inherent glands, quantity decreased significantly; After medication, rabeprazole azole group and add flavour having the above change significantly reduce in the model group, gastric mucosa structure basic integrity, epithelial structure repair, mucosal inherent glands hyperplasia is more, alignment, full size film layer, only a small amount of inflammatory cell infiltration; The rest of the group is still visible inflammatory cells infiltration, etc.(3) TNF-α:model group compared with blank group brown particles increase. Flavored having scattered groups of TNF-α significantly lower expression, brown particles significantly reduced. IL-1:model group compared with blank group increased significantly (p< 0.01), after treatment with add flavour having IL-1 expression decreased obviously (p< 0.01). PGE2:model group compared with blank expression reduced (p< 0.05), after treatment with add flavour having scattered, PGE2 expression increased (p< 0.01). Cox-2:model group compared with blank group cox-2 expression reduced significantly (p=0.05), with flavored scattered after treatment, having higher cox-2 expression (p=0.05). P-P38MAPK:model group compared with blank group P-P38MAPK expression increased significantly (P=0.05), with flavored having come loose after treatment, P-P38MAPK lower expression (P=0.05).Conclusion:Flavored Wu Qi Powder can reduce P-P38MAPK expression, promote the activation of cox-2, multiply arachidonic acid generated PG, so, reach the role of protecting gastric mucosa. And inflammatory factor expression is reduced, IL-1 indicates flavored having can reduce inflammation and promote restoration of the gastric mucosa, strengthen gastric mucosa against role of inflammatory cytokines... |
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