| Preface:With the development of the world economy, the incidence of spinal cord injury is increasing year by year. Spinal cord injury is the most serious complication of spinal cord injury, which often leads to severe functional impairment. Spinal cord injury caused a huge economic burden not only to the patient’s own physical and psychological damage, but also to the entire community. The prevention, treatment and rehabilitation of spinal cord injury has become a major problem in the medical field because of the social and economic losses caused by spinal cord injury. The health problems and social problems caused by spinal cord injury are extremely serious. The repairmen of spinal cord injury is difficult, the process is long, so it is a common medical problem at home and abroad at present. As an adjuvant treatment of spinal cord injury, hyperbaric oxygen therapy has been achieved in the basic experimental and clinical treatment, which is before the spinal cord injury, known as hyperbaric oxygen preconditioning. The effect is obvious in the experiment. By hyperbaric oxygen pre-intervention in the treatment of spinal cord injury, can effectively reduce the neuronal apoptosis occurred after spinal cord injury in the risk, reduce the injury severity, so as to give full play to its protective effect on the part and the whole of spinal cord. However, it is still clear that hyperbaric oxygen preconditioning can reduce the degree of apoptosis of the local cells after spinal cord injury and the mechanism of the effect of the apoptosis process. The purpose of this study is to further explore the mechanism.Objective:Through the study of rats after spinal cord injury were hyperbaric oxygen pre treatment processing, so as to elucidate the hyperbaric oxygen pretreatment effects on animal body movement function and of caspases in apoptosis pathway and its possible mechanism, which provided the base for the experimental researches on the deeper, and pro treatment bed application provides a theoretical basis for, and promote the development of medical level, improve the prognosis of the patients.Methods:Experimental research:animal experiment selected healthy 36 male Wistar rats, using random number table method, the rats were randomly divided into three groups:normal control group, a total of 12 rats (con, n= 12); spinal cord injury group Ⅱ, a total of 12 rats (SCI group, n= 12); (3) hyperbaric oxygen pre treatment group, a total of 12 rats (hbo-pc group, n=12). Subsequent treatment:hbo-pc group rats were 10 times hyperbaric oxygen pre treatment; hbo-pc groups of rats with SCI rats, two groups were in accordance with modified Allen’s strike method by spinal cord injury animal model in rats; (3) con-without hbo-pc treatment or any other special treatment. Model prepared 2 weeks, the following operations: 1. Rats in vivo BBB function scores and scores of records; (2) of rats in vivo, MEP detection, recorded in detail the specific data; (3) were rats, giving spinal cord tissue sections, and then remove the rat T7～T9 segment segment of the spinal cord and treated with cryopreservation; (4) were used to extract total RNA of spinal cord tissues of rats. Then the relative expression of real-time fluorescence quantitative PCR method for detection of spinal cord tissue caspase-12, caspase-7, Caspase-8, Caspase-3, Fas, TNF alpha and TNF-R Ⅰand TNF-R Ⅱ, CYC, Bak, Bax and Bcl-x and bcl-2.Results:Experimental study:Compared with the CON group, the expression levels of CYC, Caspase-12, Caspase-7, Caspase-8,, Bak, Caspase-3 and Bax were significantly higher in the spinal cord tissues of the rats in the group HBO-PC and the group SCI (P＜0.01). The BBB motor function scores and MEP test results in rats of the HBO-PC group are significantly higher compared with the SCI group rats, the difference was statistically significant (P＜0.01). No obvious spinal cord injury was found in the MRI of the HBO-PC group. Compared with the CON group, the SCI rats spinal cord tissue structural disorder, gray matter and white matter boundary is not clear, reduce neuron and microvascular, nerve fiber disruption, obvious glial scar and microcapsule structure formation;the HBO-PC group rat spinal cord tissue Nissl bodies decreased significantly, mesenchymal cells are not uniform, gray matter and white matter structure relative the SCI group is clear, visible Microcystis formation. Compared with the SCI group and the HBO-PC group, the expression of Caspase-12, Caspase-7, Caspase-8 and Caspase-3 were decreased, the difference between the two groups has statistical significance (P＜0.05); expression level of Bak was increased, the difference between the two groups has no statistical significance(P>0.05); the expression level of CYC and Bax was decreased, the difference between the two groups has statistical significance(P＜0.05). The expression of Bcl-x, Bcl-2, Fas, TNF-α, TNF-RⅠ and TNF-RⅡ in the CON group, the HBO-PC group and the SCI group was not statistically significant(P>0.05).Conclusion:This experiment of HBO-PC before SCI in rats can produce protective effect on local tissue injury. In the final analysis, since given HBO-PC after SCI in rats can inhibit the expression of caspase-12, caspase-7, Caspase-8, Caspase-3, CYC, Bax and other pro apoptotic factors, effectively reduce the number of neuronal cell apoptosis, reduce the apoptosis of endoplasmic reticulum and the mitochondria pathway, so as to reduce the degree of damage, so the specific mechanism may related to this. And HBO-PC has nothing to do to reduce neuronal apoptosis and cell death signaling pathway. |
PDF Full Text Request