Effect Of Ginsenoside Rh2 On The Migration And Metastasis Of Colon Cancer LoVo Cells And The Mechanism Of Its Action

Research background and purposeColorectal cancer (CRC) is a common occurrence in the colon of malignant tumor of digestive tract, as in recent years, people’s living standards improve, the influence of diet structure, radiation injury, obesity, smoking, genetic algorithm, and so on many factors, the incidence of colon cancer in China rate in rising stage, at present in most regions of China has become one of the fastest rise in the incidence of malignant tumors. Colon cancer once face surgery, chemotherapy, or even the occurrence of cancer metastasis, and a high rate of recurrence, postoperative survival time is short, in view of this situation, how to in colon cancer early active treatment and prevent cancer metastasis and improve operation of colon cancer patients survival rate, prolong the survival time has become one of the hot topic in the current research.Ginseng is already in clinical application in China more than two thousand years of traditional Chinese medicine of Invigorating Qi herbs, is the root of Araliaceae plant ginseng (Panax ginseng C. A. Mey.), the drug is sweet, slightly bitter, lukewarm, owned by the lung, spleen, Heart Sutra; a nourishing qi, invigorating the spleen and tonifying the lung, Sheng Jin, soothe the nerves Zhiyi, fill various dirty air, anti fatigue, relieve brain fatigue effect. Ginsenoside as the main active ingredients in ginseng, the cancer cells have obvious anti invasion and metastasis, can be combined with the operation after taking, enhance the speed of wound healing and physical recovery after operation. Ginsenoside Rh2 is the main active ingredient of ginseng, which can inhibit the growth, proliferation and metastasis of tumor cells, and induce the apoptosis of the tumor cells. Ginsenoside Rh2 combined with chemotherapy can improve the therapeutic effect and reduce the side effects.This study aimed to from cellular level and molecular level to explore the effect of ginsenoside Rh2 on human colon colon cancer LoVo cell migration and metastasis ability influence, on the one hand to elucidate effect of ginsenoside Rh2 on human colon cancer LoVo cell viability, proliferation, apoptosis, migration and metastasis of the effects and related mechanism of action to provide experimental basis; on the other hand for ginsenoside Rh2 treated human colon cancer and prevent the metastasis of cancer cells to provide experimental pharmacological basis.MethodsThe cytotoxicity of ginsenoside Rh2 was detected by MTT colorimetry. With different concentrations of ginsenoside Rh2 treated human colon cancer LoVo cells. By MTT colorimetry to detect Rh2 on LoVo cell toxicity impact than. According to the results determined will eventually be used to study the inhibitory effects of LoVo cell migration and metastasis ability of Rh2 concentrations. According to inhibit moderate drug dose and action time of the drug, respectively, set up the blank control group and the experimental group (5,10,20,40,60μmol/L), by scratch test and effect on LoVo cell scratch dosing for 24 h were observed after different drug concentrations on cell migration; by Transwell assay observe effects of different concentration of ginsenoside Rh2 on cell metastasis; Western blot method for the detection of different drug concentration of Rh2 on CD44, matrix metalloproteinase MMP-2 and matrix metalloproteinase tissue inhibitor of metalloproteinase-2 (TIMP-2), E-cadherin and-Catenin above have differential gene expression detection of changes in the level of protein expression..Results1. The MTT results showed that compared with the control group, the concentration of 5,10,20,40 μmol/L of Rh2 processing LoVo cells 24 hours after, treatment group cell growth was significantly inhibited, the cell survival rate showed no significant difference (P>0.05). When Rh2 concentrations greater than or equal to 60 μ mol/L for 24h, the concentration is more than or equal to 40 mol/Ltreatment 48h, the concentration is greater than or equal to 20 μ mol/L treatment for 72 h, LoVo cell growth was significantly inhibited, the difference is statistically significant (P< 0.05), prompt effect of ginsenoside Rh2 on LoVo cell viability has time and concentration dependent. The concentration of Rh2 in 0 to 40μmol/L in the range of LoVo cells treated with 24h on LoVo cells neither cytotoxicity nor proliferation. However, with the prolongation of Rh2 time and the increase of concentration, the inhibition effect of Rh2 on LoVo cells was enhanced. So in the following experiments, the effect of the concentration of Rh2 control in the 40μmol/L, the role of time for the study of 24h.2. Scratch wound assay and Transwell results show that, its inhibitory effect on LoVo cell migration and metastasis ability increased with the increase of ginsenoside Rh2 drug concentration, more than 20μmol/L concentration of Rh2 processing LoVo cells in 24h and the control group compared with significantly inhibited (P<0.05).3. Westernblot results showed that with the increase of Rh2 drug concentration, CD44, MMP-2 protein expression levels decreased, TIMP-2, E-Cadherin and beta-catenin protein expression levels increased.Conclusions 1. Ginsenoside Rh2 can inhibit LoVo cell viability and increase its value, its role has a time and concentration dependent.2. Ginsenoside Rh2 has inhibitory effect on cell migration and metastasis of LoVo cells. The effect is time and concentration dependent.3. Ginsenoside Rh2 can down regulate the expression level of CD44 and MMP-2 protein, and up regulate the expression level of TIMP-2, E-Cadherin and beta-catenin protein.