Intervention Effect Of Curcuma Kwangsiensis Polysaccharides(CKP)on Type 2 Diabetes Mellitus Rats

Objective: Investigate the effect of Curcuma Kwangsiensis Polysaccharide(CKP) intervention on rats with type 2 diabetes mellitus(T2DM) by observing the blood sugar, fat, oxidation markers of pancreas, the morphologic changes of pancreas and the protein expression of Fas in islet cells, and then explore the anti-diabetic effect and mechanism of CKP. Methods: T2 DM models were induced through high-sucrose and high-fat diet combined with low-dose abdominal injection of streptozotocin(STZ, 45mg·kg-1). The successfully modeled rats were randomly divided into 4 groups: T2 DM model group,CKP low dosage group(CKP-LG), CKP middle dosage group(CKP-MG) and CKP high dosage group(CKP-HG). Another 10 normal rats were selected as normal group. CKP groups with different dosages(0.5, 1.0, 2.5g·kg-1·d-1) were ig administered continuously for 6 weeks, once a day. The model group and normal group received ig normal saline of the same volume. After 6 weeks of administration, fasting blood glucose(FBG), insulin(INS), total cholesterol(TC), triglyceride(TG) and the contents of SOD, MDA, GSH-Px, CAT inpancreas tissue levels were detected in rats, and pathological change of pancreas was evaluated by hematoxylin and eosin(HE) staining and immuneohistochemistry, Fas protein expressions of pancreatic β cells were detected.Results: Compared with the normal rats, FBG, TG, and TC levels were increased significantly while the levels of the SOD, MDA, GSH-Px, CAT decreased obviously in rats of model group, and pancreas Fas protein expressions were increased, with statistically significant difference(P<0.05,P<0.01), Compared with the model group, CKP groups could reduce FBG, TC and TG levels of T2 DM rats, as well as improve the levels of SOD, MDA,GSH-Px, CAT, but reduce pancreas Fas protein expressions and reduce apoptosis of pancreatic β cells, with significant difference(P<0.05, P<0.01).Conclusions: The intervention effect of CKP on T2 DM rats may be achieved by adjusting the antioxidant stress and protecting pancreatic β cells and reducing their apoptosis.