Regulation Of Xiaoyusanjie Enema Of Tumor Suppressor To Gene PTEN Expression On A Prostate Cancer Model In Nude Mice

ObjectiveProstate cancer(PCa) is a common malignant tumor in male genitourinary system, it incidence keep rising for several years in China. In our country most prostate cancer confirmed at the middle-latest age of the tumor and can not be cured by radical prostatectomy. The part of patients can benefit from endocrine therapy at the beginning of the treatment, but all of them will develop castrate-resistant prostate cancer (CRPC) after a median time of 1 or 2 years. Now, there is not a standard and effective treatment for CRPC at home and aboard, but the traditional Chinese medicine treatment of CRPC has got certain affirmation.In this study, to study if xiaoyusanjie enema of tumor suppresscor can inhibit nude mice bearing human prostate carcinoma and to explore its molecular mechanisms, as so to lay a good foundation for the application of anti-cancer traditional Chinese medicine.MethodsHuman prostate carcinoma PC-3 cells were cultured in vitro. They were inoculated to 30 nude mice at the right forelimb extremity near the back and neck. Then the mice were randomly divided into treatment group(n=15) and control group(n=15). After 10 days of the inoculation, the treatment group was treated by xiaoyusanjie enema of tumor suppressor once every two days for 30days and the control group were with same volume of saline in the same way. During the period of treatment, we measured the tumors size and the weight of mice, observed the condition of the mice. At the end of the experimentation, the tumors were dislodged completely then we measured the size of tumors, drawed the grow the curve of the tumors, calculated the inhibition rate of the tumor and detected the expression the PTEN with the method of qRCR and Western Blot.Results1.The volumes of tumor in nude mice of treatment group was less than the control group (P<0.05) after the treatment of 30 days by Xiaoyusanjie enema of tumor suppressor. The inhibition rate to the tumor growth was 21.8%.2. Mesured by RT-qPCR, the relative expression of the PTEN mRNA was higher than the control group (P<0.001). And compared with the control group was statistical significantly difference. Western blot method showed the protein expression of PTEN in treatment group was (0.55±0.13), and the control group was (0.32±0.09). The difference was significant(P<0.001).Conclusion1. Xiaoyusanjie enema of tumor suppressor have certain inhibitory response on nude mice bearing human prostate carcinoma.2.UP-regulationof the expression of PTEN gene and protein translation maybe one of the mechanisms through which xiaoyusanjie enema of tumor suppressor induced growth inhibition of nude mice bearing human prostate carcinoma. Thus inhibit PC-3 cell proliferation.