Study On BRMS1 Gene Transfection Of Human Pancreatic Cancer Cells In Nude Mice Effects Of Tumor Metastasis

BackgroundIn the 21st century, with the rapid development of the global economy, occurrence of the corresponding human disease spectrum changes, maligant tumor of the threat to human health is increasingly prominent. Currently, pancreatic cancer has become the 4th leading cause of death in the disease, its incidence has significantly increased the trend. Because of pancreatic cancer earlier in the blood and lymph node metastases occur, leaving early diagnosis is difficult, and a high mortality rate, pancreatic cancer patients are diagnosed after a median surial of no more than 6 months, the 5-year survival rate less than 5%. Therefore, further study invasion and metastasis of pancreatic cancer molecular biology mechanism, to improve the curative effect of pancreatic cancer will play an important role. In recent years, with the deepening of the research of molecular biology, the mechanism of tumor metastasis have been gradually recognized, Study found tumor progression to metastasis needs at least two genes, namely metastasis gene and anti-metastasis gene. Inhibit tumor metastasis to treat cancer can be taken into the consideration of, will become a new way to treat cancer. Breast cancer metastasis suppressor1(BRMS1)is abroad recently reported tumor metastasis of pancreatic cancer suppressor genes, transfer inhibition of research BRMS1 potentially application value.While BRMS1 gene does not affect primary tumor growth, but can inhibit tumor cells to distant metastases, as a newly discovered metastasis suppressor gene therapy for pancreatic cancer has a good prospect.ObjectiveThe establishment of human pancreatic cancer nude mice model to study the BRMS1 gene in human pancreatic cancer cells in nude mice tumor metastasis ability to find an effective model of pancreatic cancer gene therapy.Methods Liposome transfection technique using the constructed pEGFP-C1-BRMS1 recombinant vector, pEGFP-C1 empty vector stably transfected into human pancreatic cancer cell line PANC-1, the inoculated three groups (transfected group, empty vector group, not transfected group) cells subcutaneously in nude mice, mice were killed after 5 weeks, the tumor, lymph node and lung tissue for histopathological examination.ResultsThe pEGFP-C1-BRMS1 recombinant vector, pEGFP-C1 empty vector transfected into human pancreatic cancer cell line PANC-1, and established human pancreatic cancer xenograft model in nude mice, three groups no significant difference in tumor size in nude mice. However, lymph node metastasis in nude mice transfected group was significantly lower than the empty vector group and untransfected group.ConclusionBRMS1 gene in human pancreatic cancer cells without affecting tumor growth in nude mice subcutaneously, but can inhibit human pancreatic cancer cells transplanted into nude mice of the local lymph node metastasis.