Preparation And Drug Properties Of Fe₃O₄ Nanocomposites

Objectives:1. In order to enhance the stability and the biological compatibility of Fe₃O₄ magnetic nanoparticles（MNPs）, and the Fe₃O₄ particles were modified by the polymer compound chitosan（CS） and polyethylene glycol（PEG）, which the polymer / magnetic nanoparticle system formed after the modification was more extensive in use and function.2. Then using doxorubicin as drug carrier studied in vitro load performance of drug for the composites, and the effect of drug loading on the in vitro activity of the cells was studied.Methods:1.（1） In order to enhance the biocompatible and biodegradable of Fe₃O₄ magnetic nanoparticles and make it get more extensive application, Fe₃O₄ was modified by non-toxic natural polymer chitosan. Chitosan-Fe₃O₄ magnetic nano composites was synthesized by using glutaraldehyde as crosslinking agent.（2） Polyethylene glycol polymer has non-toxic,high water solubility and good biocompatibility. Polyethylene glycol / Fe₃O₄ magnetic nano composites was synthesized by one-step. Through the surface modification of Fe₃O₄ nanoparticles, the defects of Fe₃O₄ will be effectively improved.2. Two kinds of magnetic nano composites with adriamycin were included by ultrasonic method. The encapsulation efficiency and drug loading of the composite particles on doxorubicin were determined by ultraviolet- visible spectrophotometry（UV-vis）, and the in vitro drug release of the inclusion compound was investigated.3. Two kinds of magnetic nano composites with adriamycin on esophageal cancer cell growth were detected by MTT colorimetric method.Results:1. PEG and CS had successfully combined with Fe₃O₄, which forming two different types of magnetic nanocomposites.2. PEG/Fe₃O₄ and CS-MNPs had good inclusion property and drug release properties of the DOX.3. PEG/Fe₃O₄ and CS-MNPs with adriamycin can effectively inhibit the proliferation of K150 esophageal cancer cells.Conclusions:1. Synthesis of the two magnetic nanocomposites had good morphology and performance.2. Two magnetic nanocomposites had a good effect on DOX, and the drug release mechanism of the inclusion compound was in line with our requirements.3. The synthesis of inclusion complex can effectively inhibit the propagation of cancer cells, indicating that the synthesis of CS-MNPs and PEG/Fe₃O₄ was good, and had no effect on the efficacy of DOX.