| Coronary heart disease as one of the most common cardiovascular diseases has been a serious threat to human health.At present,implanting the intravascular stent in the area of the disease to unblock the blocked arteries is the most effective method.However,stent graft can cause damage to the vascular wall when it is implanted,and lead to complications such as stenosis and thrombosis.The recent research is building biological function in the surface of stent graft to make stent can quickly form a layer of endothelial after implantation in the human body,and accelerate the repair and reconstruction of endodermis damage,thus solving the problem of the postoperative complications.In this paper,the dopaminergic coating was deposited on the surface of the metal titanium foil,and it was grafted with the thiophenol chitosan/polyethylene glycol diacrylate composite hydrogel.By means of the reaction condition,the content of the crosslinking agent,the concentration of the monomer were screened by water gel synthesis method.We evaluate hydrogel through a series of representational such as the characteristics of morphology,structural characteristics,solubilitiation,degradation performance and endothelial cell compatibility.This thesis evaluated the monomer synthesis and characterization results.the synthesis of sulphur chitosan(TCS)experiments show that change of thioglycolic acid and chitosan ratio,reaction time and the dosage of the two imine carbide can control sulphur chitosan thiol degrees,in this experiment of sulfhydryl chitosan reaction conditions as follows:the reaction system pH = 5.0,EDC concentration 0.3 mol/L,thioglycolic acid and chitosan material ratio(mass ratio)of 1:1,the reaction time for 10 h.Polyethylene glycol diacrylate(PEGDA)synthesis experiments showed that under the triethylamine and argon gas protection system,the synthesis of PEGDA reaction was comfirmed successful preparation by infrared detection and nuclear magnetic resonance hydrogen spectrum.In the screening of hydrogel synthetic conditions,this thesis by means of the reaction conditions,concentration of monomer,crosslinking agent.Through the gel conditions selected TCS-PEGDA composite hydrogel reaction conditions.concentration of sulphur chitosan(TCS):20mg/mL,concentration of polyethylene glycol diacrylate(PEGDA)100mg/mL,concentration of ammonium persulfate(APS):20 mg/mL,tetramethyl ethylene diamine(TMEDA)amount to 20μL.This thesis characterize the properties of TCS-PEGDA(TP)composite hydrogels,the SEM resulted the more PEGDA content,the lower ydrogel inside porosity,and material become more dense;Hydrogel FTIR experiments confirmed that the synthesis of hydrogels have characteristics of groups,the experiment confirmed the TP hydrogels was prepared successful;Hydrogel swelling performance experiment confirmed as PEGDA content increased,the hydrogel swelling performance became lower;Hydrogels degradation performance experiments confirmed that with the increase of content of PEGDA,hydrogels degradation rate was flat,and the degradation rate of TP2 sample group was obviously higher than that of the other three groups.Endothelial cell adhesion experiments show that the hydrogel has good cell compatibility,with the increase of sulphur chitosan,the better the performance of cell adhesion;HE dyeing experiments showed that cells can be migrated from the hydrogel surface into the hydrogel inside.To sum up,this thesis synthesized the sulfhydryl chitosan and polyethylene glycol diacrylate composite hydrogel,and evaluate the evaluate through the topography characteristics,structural characteristics,swelling properties,degradation of performance,endothelial cell compatibility,.finally,this thesis concluded that the TP4 composite hydrogel group has good swelling performance,degradation performance and endothelial cell compatibility. |
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