Roles Of Calcium-sensing Receptor And Its Related Factors In Pathogenesis Of Persistent Pulmonary Hypertension In Neonatal Rats

Object: To investigate the expression of CaSR in the lung tissue of neonatal rats.To explore the roles of calcium-sensing receptor(CaSR) and its related factors in the lung tissue of neonatal rats in the pathogenesis of persistent pulmonary hypertension(PPH),to learn more about the pathogenesis of persistent pulmonary hypertension(PPH) in neonatal rats.Methods: Use intrauterine hypoxia and intraperitoneal injection of indomethacin to make a neonatal rat model of PPH.Six Sprague-Dawley(SD)rats on the 19 th day of pregnancy were randomly divided into PPH and control groups(n=3 each). The PPH group was injected with indomethacin(0.5 mg/kg) intraperitoneally twice daily and exposed to 12% oxygen for three days. The control group was injected with an equal volume of normal saline intraperitoneally and exposed to air. Neonatal rats were born by caesarean section from both groups on the 22 nd day of pregnancy.The m RNA levels of CaSR and type II 11β-hydroxysteroid dehydrogenase(11β-HSD2)expression in the lung tissue of neonatal rats were observed by RT-PCR,and the expression of CaSR protein was observed by Western blot,in ddition, the content of nitric oxide(NO)was detected.Results(1)Compared with the control group,the thickness of the right ventricular free were higher,and the ratio of media/(media+lumen) thickness was also higher.(2)Compared with the control group,The levels of mRNA and protein of CaSR in the lung tissue in the PPH group were higher,the content of NO was also higher,while the levels of m RNA of 11β-HSD2 in the lung tissue were lower in the PPH group.Conclusion CaSR and its related factors may play important roles in pathogenesis of PPH in neonatal rats.