The Correlation Of BRAF^V600E Point Mutations And Ki-67 Protein Expression In Papillary Thyroid Carcinoma

Objective: To explore the clinicopathological significance in papillary thyroid carcinoma through examining and comparing expressed ki-67 and BRAF^V600E mutation in cancerous and adjacent normal thyroid tissues, as well as the impact of expressed ki-67 and BRAF^V600E mutation on the diagnosis, treatment and prognosis of papillary thyroid carcinoma.Methods:（1） We chose 73 cases of papillary thyroid carcinoma and adjacent normal thyroid tissues 10cases; all of samples were the formalin-fixed and paraffin embedding tissues.（2） Immunohistochemistry（Envision method） was used to detect the expression of ki-67; the mutant allele specific amplification method（MASA-PCR） was used to detect the BRAF^V600E point mutation;（3） We collected 73 cases of clinical data and analysed their clinical pathological characteristics;（4） Statistical analysis was performed using SPSS（version 17.0） statistical software package. Qualitative data were analyzed using chi-square test,Spearman rank correlation test and Kappa consistency detection was used to analyze the correlation between ki-67 expression and BRAF^V600E mutation in thyroid carcinoma and its clinical pathological significance.Results:（1） There was no expression of ki-67 and BRAF^V600E mutation in adjacent normal thyroid tissue;In papillary thyroid carcinoma, ki-67 low expression and BRAF^V600E mutation rate was high, we considered that BRAF^V600Emutation and the expression of ki-67 protein could be as a index of diagnosis and treatment of papillary thyroid carcinoma.（2） The expressions of ki-67 in papillary thyroid carcinoma tissues become significantly higher along with the cancer focus to lager, the extracapsular invasion, lymph node metastasis,bilateral thyroid carcinoma.The BRAF^V600E mutation were correlated with cancer focus, lymph node metastasi, the extracapsular invasion and clinical stage. ki-67 expression and BRAF^V600E mutation prompted with papillary thyroid carcinoma development, invasion and metastasis.（3） There was relationship between ki-67 protein expression and BRAF^V600E mutation in papillary thyroid carcinoma（P <0.05）, both could be as clinical index of judging the prognosis of patients with papillary thyroid carcinoma.（4）Comparison group were using immunohistochemical method to detect BRAF^V600E mutation, the result of the two kinds of detection methods have better degree agreement.Conclusions:（1） The BRAF^V600E mutation is correlated with the clinical pathological progress of papillary thyroid carcinoma and poor prognosis;ki-67 protein expression with papillary thyroid carcinoma is closely related to the ability of invasion and metastasis;（2） There is relationship between the BRAF^V600E mutation and ki-67 protein expression of four groups in papillary thyroid carcinoma tissues,prompts both detection can be used as papillary thyroid carcinoma diagnosis, treatment and prognosis judgement index.