| Introduction：To explore the mechanism of whole-body vertical vibration therapy onpostmenopausal osteoporosis and provide reference for clinical treatment ofpostmenopausal osteoporosis, this paper investigated the effects of whole-body verticalvibration on the generalized parameters (body weight, uterus weight, intra-abdominal fatweight), bone mineral density, serum E2level, bone microstructure, bone biomechanicalproperties and the protein expression of FoxO1in tibia and bone marrow cell ofovariectomized osteoporosis rats.Methods：Thirty-six healthy3-month-old female SD rats were randomly divided into thefollowing three groups by body weight: sham-operation (Sham) group, ovariectomized(OVX) group, and OVX whole-body vertical vibration (OVX+Vit) group. At11thweek ofoperation, the OVX+Vit group rats were vibrated on a vibration platform twice per day for7weeks according to the following schedule: whole-body vertical vibration for15min,and take a rest for10min. The frequency is90Hz and the amplitude is0.5mm. After7weeks of whole-body vertical vibration treatment, the serum, uterus, intra-abdominal fat,legs and the fifth lumbar vertebra were isolated. Then detect the uterus weight andintra-abdominal fat weight by electronic scales, detect the serum E2level byradioimmunoassay, detect the bone mineral density by DEXA, detect the bonemicroarchitecture and bone histomorphometry by micro-CT, detect the bone biomechanicsof the fifth lumbar vertebra and femur by electronic universal material testing machine.Grinding to extract protein of right tibia, take the bone marrow cell of right femur thendetect the protein expression of FoxO1by western blot.Results：After the operation, the body weight of OVX group was significantly higher thanthat of Sham group, the uterus weight in OVX group was significantly lower than that inSham group. After the whole-body vertical vibration, the added body weight after trainingin OVX+Vit group was significantly lower than that in OVX group. The uterus weight andthe intra-abdominal fat weight had no significant difference between OVX+Vit group andOVX group. As revealed by the results of bone mineral density: The femur and lumbar ofOVX group was significantly lower than that of Sham group; the femur and lumbar ofOVX+Vit group were significantly higher than that of OVX group. The serum E2level ofOVX group was significantly lower than that of Sham group, but the serum E2level ofOVX+Vit group was no significant difference than that of OVX group. As revealed by theresults of micro-CT, the vBMD, BV/TV, Tb.N and Tb.Th in both distal femur and the fifthlumbar vertebrae of OVX group were significantly lower than that of Sham group, whereasthe Tb.Sp and SMI were significantly higher than that in Sham group. The vBMD and Tb.N in distal femur of OVX+Vit group was significantly higher than that of OVX group,while the Tb.Sp in OVX+Vit group was significantly lower than that in OVX group,however, there was no difference in the Tb.Th and SMI between OVX+Vit group andOVX group. There were no difference in all detected parameters of the fifth lumbarvertebrae between OVX+Vit group and OVX group. As revealed by the results ofstructural mechanics performance index, the maximum load, elastic load maximumdeflection and fracture load in femur of OVX group were significantly lower than that inSham group; the maximum load, maximum deflection and fracture load in femur ofOVX+Vit group were significantly higher than that of OVX group. The fracture load andmaximum deflection in lumbar vertebrae of OVX group were significantly lower than thatof Sham group; the fracture load in lumbar vertebrae of OVX+Vit group was significantlyhigher than that of OVX group. With the material mechanics performance index, themaximum stress, elastic stress and elasticity modulus in femur of OVX group weresignificantly lower than that of the Sham group; there were no difference in all detectedparameters of the femur between OVX+Vit group and OVX group. The fracture load andmaximum deflection in lumbar vertebrae in OVX group were significantly lower than thatin Sham group; the breakdown strain in lumbar vertebrae of OVX+Vit group wassignificantly higher than that of OVX group. The protein expression of FoxO1in tibiaorganization and bone marrow cell: There was no expression of FoxO1in tibiaorganization, but expressed in the bone marrow cell. Compared with Sham group, FoxO1protein expression level had no significantly difference in bone marrow cell of OVX group;compared with the OVX group, the protein expression of FoxO1in bone marrow cell wassignificantly reduced of OVX+Vit group.Conclusion: Whole-body vertical vibration could inhibit the increasing of body weight,increase the bone mineral density of femur and lumbar in ovariectomized osteoporosis rats,but couldn’t influence the serum E2level. Whole-body vertical vibration could improve thetrabecular bone microarchitecture of the femur in ovariectomized rats, optimize thestructure of bone, but the whole-body vertical vibration could not improve the trabecularbone microarchitecture of lumbar. Whole-body vertical vibration could improve the bonebiomechanics of femur and lumbar on ovariectomized rats. Whole-body vertical vibrationcould reduce the protein expression of FoxO1in bone marrow cells. There was no proteinexpression of FoxO1in tibia. |
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