Research Of The Effect Of Aerobic Exercuse On Enzyme Activity Of Energy Metabolism In Passive Smoking Mice

Objective: this study through the establishment of passive smoking of mice and the model of low intensity aerobic exercise, preliminarily exercise intervention of passive smoking on mice liver tissue and muscle aerobic capacity, the nfluence of exploring low intensity aerobic exercise model of passive smoking of mice active enzyme activity related to the liver, muscle, the effects of passive smoking for aerobic exercise intervention study provides reference.Methods: use 2 months of age 36 kunming mice, were randomly divided into 4 groups: quiet group(group C, 8), smoking group(group S, 10), exercise group(group E, 8) smoking and exercise group(SE group, 10).S, and SE group separately put in homemade semi-closed glass, choose the static type of passive smoking exposure edify.Burning cigarettes for a jiangxi ordinary cigarettes, cigarette factory production with 10 mg/tar, carbon monoxide content 4 mg/branch, nicotine content of 0.8 mg/teams. Passive smoking for smoking 2 times a day, every time 90 minutes, passive smoking at 9:00 am and PM, 5 minutes each cigarette burning, change once every 15 minutes, 12 a day, six days a week, for 2 weeks.Smoke box specifications for 60 cmx40cmx40 cm, side is equipped with sliding door leave gaps.In the process of passive smoking, canister to the side of the tank design eight holes, ensure air exchange with the outside world.The rest of the group not to passive smoking, quiet after 2 weeks;Incremental 4 weeks after aerobic exercise with independent way of swimming, swimming time each day for the PM, 6 days a week.Choose 1 m barrels deep, diameter of 80 cm of homemade swimming equipment, material for the deep translucent white plastic barrels, barrels around the inner wall is smooth.After six weeks, the mice with ether anesthesia eyeball blood, in order to extract the liver and bone quadriceps group, weighing.With colorimetric method for liver tissue, PK and CK LDH, MDH, SDH and the activity of serum BUN and quadriceps Ca2 +- magnesium- ATP, Na +-k +- ATP levels.Using SPSS18.0 variance analysis of the indicators. The results of the study:2 weeks smoke canister, 4 weeks aerobic exercise after smoking, smoking group(movement), using the mathematical statistics compared between groups, the results showed that:(1) Passive smoking 2 weeks later, the mouse hair yellow, upside down, slow, immobile, spirit is tired and lazy.Smoking group(S) weight of mice significantly lower in the quiet group(P < 0.05).Show that passive smoking can affect the body metabolism, make the body weight growth is slow.Aerobic exercise after 6 weeks, quiet group(C) weight compared with smoking group(S), no significant difference(P > 0.05).But movement smoking group(SE) and exercise groups(E) have been losing weight, SE exists significant difference(P 0.05) or less;Smoking group(S), the weight had no significant difference(P > 0.05).(2) After the passive smoking of aerobics exercise load index changes.Compared with group C, group E lower serum BUN content, higher SE group, there are significant differences(P < 0.05);Compared with group C, the rest of the group of liver CK activity increased slightly, but there was no significant difference(P > 0.05).(3) Passive smoking for glycolysis metabolic enzyme changes after aerobic exercise.Compared with group C, group E liver PK active enzyme increased slightly, but no statistically significant differences between groups.Compared with group C, group E liver LDH activity enzyme slightly lower, SE group was higher, but no statistically significant differences between groups.(4) Passive smoking and aerobic metabolism enzyme changes after aerobic exercise.SDH content compared with group C, group a slight growth, there was no significant difference;MDH levels compared with group C, group E rise, very significant differences(P < 0.001), SE compared with group C, the significant difference(P < 0.05).(5) The passive smoking and the changes of atpase after aerobic exercise.Compared with group C, calcium, magnesium, sodium, potassium- atpase slightly increases, the potassium sodium- atpase, group E significant differences compared with group C(P < 0.05).Compared with group C, the rest of the group was no significant difference(P > 0.05). Conclusion:(1) Smoke exposure can reduce weight gain in mice.(2) 4 weeks aerobic activity of metabolic enzymes of glycolysis system had no significant influence.(3) 4 weeks aerobic exercise, the body more resilient aerobic, however, sports group, the amplitude of MDH increased smoking results showed that the sports can improve aerobic metabolism enzyme activity, short-term smoke exposure inhibiting liver aerobic metabolism enzyme activity.However, 4 weeks aerobic exercise on the glycolysis system no significant metabolic enzyme activity.(4) The aerobics potassium sodium- atpase activity increases, passive smoking inhibit ATP enzyme activity.