Aerobic Exercise And Supplement The Effect Of MT On The Antioxidant And ATPase Activity Of Passive Smoking Rats

Objective: In order to investigate the aerobic exercise and supplement the effectof MT on the antioxidant capacity of passive smoking rats, the experimental model isfirst built passive smoking, then observe the effect of supplementation and exercise onrats.Method: First select the February age32healthy male SD rats were divided intoexercise, passive smoking group (ES), passive smoking group(S), exercise group (E),control group (C), is used to model. ES, E groups were12weeks of strength inswimming training, ES, S, passive smoking, the dose of8/group/day. SerumT-AOC, Ca2+, BUN levels were measured by using semiautomatic spectrophotometer;rat lung tissues were SOD, MDA, GSH-PX enzyme activity; learning performancethrough digital X-ray imaging system to observe the pulmonary imaging. Then selectthe February old healthy male rats64, divided into the quiet group (C), exercise group(E), smoking group (S), medicine tonic group(MT), exercise smoking group(ES),medicine tonic exercise group (MTE), medicine tonic smoking group (MTS),medicine tonic exercise smoking group (MTES). ES, MTES, E, MTE four groups ofrats have aerobic exercise; for8weeks; MTS, MT, MTES, MTE groups are tested bygarage at a dose of0.5ml/100g body weight. Water maze was used to observe thechanges of learning and memory ability of rats. The last movement, passive smokingand the intervention of MT.HE staining was used to observe the lung, the charge ofblood GSH-PX, CAT, SOD spectrophotometer, MDA enzyme activity and lung,skeletal muscle ATP activity meter.Result:（1）From the model rats of imaging observation, on4th,8th,12th’s weekendBilateral middle and lower lung field markings, thickening, fuzzy and with theextension of time effect in rat lung get more serious. Results showed that4weeks of passive smoking can cause lung pathological changes.(2) From the model rats,12weeks of passive smoking induced oxidative stresspassive smoking will make the serum Ca2+content decrease and in the intervention ofaerobic exercise the serum Ca2+content increase, aerobic exercise and passivesoaking’s Intervention make the serum BUN of rats increased significantly.(3)4weeks of passive smoking will delay the weight and lung tissue growth anddevelopment of the body, the supplement of exogenous MT can improve the growthperformance of passive smoking rats, aerobic exercise can stimulate lung growth andchanges of the organization.（4）The change of serum and tissue’s antioxidant enzyme activities of rats in eachexperimental group is significant, GSH-PX, CAT, SOD, MT, ATP enzyme activity ofaerobic exercise and its interaction when the enzyme activity is increased, the passivesmoking intervention when the enzyme activity was significantly decreased, but theMDA enzyme shows the opposite trend.Conclusion:12weeks of passive smoking induced rat growth performance significantly andserum Ca2+content, antioxidant enzymes activity decreased.4weeks of static modelof passive smoking can cause lung pathological changes and12weeks aerobicexercise can increase the contents of serum Ca2+and BUN levels, delay of passivesmoking induced oxidative stress injury.4weeks of passive smoking affects decreases antioxidant capacity of organismand impair learning and memory ability,8weeks of regular aerobic exercise andsupplement of MT delay tissue damage, increasing antioxidant enzyme activity peroxidation of lipid reducing and damage the body cells.