The Effects Of Different-intensity Exercises On The Hypothalamic Adipo- Kiss- Gnrh In Obese Male Mice

Objective: To study the effect and mechanism of an early obesity on male sexual development, the research paper is to provide experimental basis of the relationship between obesity and reproductive function. At the same time it also aims to explore a safe and effective physical exercise method and its influence mechanism,and to provide theoretical basis for exploring ways and means of prevention.Methods:. 30 pcs weaning male C57BL/6 mice were selected randomly as the high-fat diet group from 40 mice and the remaining 10 as normal diet control group(CON). After 10-week high-fat obese diet, 30 mice in the test group were randomly divided into three new groups according to body weight, namely obese control group(OB), middle-intensity exercise obese group(OB+ME) and high-intensity exercise obese group(OB+HE). 8-week swimming training was performed in the last two groups and exercise load increased gradually to 2 hours per day and 4 hours per day for each group. After training, the serum Adipo, Luteinizing hormone(LH), Follicle-stimulating hormone(FSH) and Testostrone(T) were tested with Elisa method. The genetic transcription levels of the Adipo-AMPK- Kiss-Gn RH in the hypothalamus were detected using Real-time PCR method.Results: 1. Weight, fat weight and body fat rate in OB group were higher significantly compared with CON(P<0.01 or P<0.05). Weight, fat weight and body fat rate in OB+ME and OB+HE group were lower significantly compared with OB group(P<0.01 or P<0.05). These parameters in OB+HE group were lower significantly compared with OB+ME group(P<0.05).2. The serum Adipo in OB group was lower significantly(P<0.01) compared with CON group and the serum LH, FSH and T were lower significantly too(P< 0.01). The serum adipo, LH, FSH and T in OB+ME group were higher significantly than OB group(P<0.05). These parameters in OB and OB+HE groups showed no significant difference. But these parameters in OB+HE group were significantly lower compared with OB+ME group(P<0.05).3. The hypothalamus Adipo, Adipo receptor1 and AMPK m RNA levels were higher significantly(P<0.05) and the Kiss and Gn RH m RNA levels were lower significantly(P<0.01) in OB group compared with CON group. The hypothalamus Adipo, Adipo receptor1 and AMPK m RNA were lower significantly(P<0.01 or P<0.05) but kisspeptin and Gn RH m RNA were higher significantly(P< 0.05) in OB+ME group mice compared with OB group. The levels of hypothalamus Adipo, Adipo receptor1 and AMPK m RNA were lower and Kiss and Gn RH m RNA were higher in OB+HE group than OB group but without significant differences. The hypothalamus Adipo, Adipo receptor1 and AMPK m RNA levels were higher significantly(P<0.05) and the Kiss and Gn RH m RNA levels were lower significantly(P<0.01) in OB+HE group compared to OB+ME group.Conclusion: 1. Obesity caused by an early high-fat diet regulated the hypothalamus Adipo up, inhibited Kiss-Gn RH gene transcription and the function of hypothalamus- pituitary- gonad(HPG) axis and thus affected the start of puberty.2. Long-term middle-intensity exercise reduced the body fat effectively and improved the metabolic disorder. Thus the hypothalamus Adipo was decreased and its inhibition on gene transcription of Kiss- Gn RH and the hypothalamus- pituitary- gonad axis was alleviated. So the negative effect of obesity on the sexual development was improved.3. Long-term high-intensity exercise reduced the body fat significantly and improved the metabolic disorder. But the hypothalamus Adipo was not decreased significantly so its inhibition on gene transcription of Kiss- Gn RH and the hypothalamus- pituitary- gonad axis existed. So the negative effect of obesity on the sexual development was not improved.