Research Of Paper-based Cell Culture And Real-time Dynamic Cell Growth Detection Based On Impedance Analysis

Cell culture technology is the most basic and core in biologic field. At present two-dimensional(2D) cell culture is the mainstream of biotechnology. And in recent years three-dimensional(3D) cell culture has developed rapidly. With respect to the 2D cell culture, 3D cell cultureis closer to the actual biological conditions in aspects of cell morphology, properties andculture environment. So 3D cell culture is more conducive to the study of biology. But 3D cell culture still has many technical problems, such as quantizing the number of detected cell. In this issue we developed anew three-dimensional cell culture methodto solve these problems. It's the paper-based cell culture.Compared with other 3D cell culture substrate,such ashydrogel,filter paper is mechanically movable, hydrophilic, and it has the advantage of being developed to the systems having specific function. Mechanical mobility enables the paper-based systemto have a great advantage in the detection of the cell.In this issue,for the first time,filter paper was used for the culture substrates and microfluidic culture areas and channels were patterned on the paper by wax printing technique. It could be made simply and fast. Utilizing the characteristics of the filter paper, we developed paper-based cell culture system and paper-based cell culture microfluidic system. And Analysis of cyto-compatibility, cell proliferation, cell morphology, and cell chemosensitivity were performed to confirm the possibility of the paper-based system.The real-time detection system for paper-based cell culture is developed based on the technology of paper-based cell culture and the impedance properties of the cell. The photolithography and metal vapor deposition techniques are both used in this system for making the electrode. In addition, the effective range of the electrode has been confirmed by the simulation using COMSOL. We use Princeton Versa STAT 4 electrochemical workstation to achieve impedance testing and data acquisition. Cell growth detection is usually endpoint detection, and it always takes a long time. But the system developed in this issue could detect fast and easy, and it's non-end-point detection. Compared with results of paper-based ELISA test, the accuracy of the cell impedance detection system could be verified. In conclusion paper-based cell culture and real-time dynamic cell growth detection based on impedance analysis is feasible.