Development Of The Colloidal Gold Test Paper Strip For The Detection Of Deoxynivalenol

As a common fungal toxin,Deoxynivalenol exists in all countries of the world to have serious pollution.In our country,the content of grain is more serious than the standard.At present stage,DON detection technology in TLC,ELISA and HPLC method can quantitative analysis,but its cumbersome operation,can not achieve rapid detection of the field,and the development of the test of colloidal gold immunoassay test strip is more convenient,suitable for a large number of samples of the rapid detection.This paper determinated by two methods,comparison of ascorbic acid and sodium citrate,the gold particles prepared by ascorbic acid is not easy to preserve,the stability is poor,and ultimately determine by 2 mL of concentration of 1% sodium citrate to restore the 0.01%HAuCl4,the gold particles size is about 17 nm.Through continuous optimization,1 mL colloidal solution of gold to add 0.1 mol/L K₂CO₃ solution 17 ?L,DON McAb is 5 ?g and stabilizer to add 100 ?L 1% PEG,add 10% BSA 100 ?L,centrifugation after precipitated at the bottom with PBS suspending,gold labeled antibody with complex solution diluted 5 times,detection line and a quality control line respectively by 0.4 mg/mL DON-BSA and 0.6 mg/mL anti-mouse antibody,NC membrane models for CN140,drying for 4 hours at 37?,the coated liquid 0.01mol/L pH7.4PBS?5% sucrose and 5% methanol?join 0.085 g/L of 10% NaCl to enhance the effect of color.The sample pad type is GL-b03,the conjugate pad type is GL0194.Sample pad of the treating liquid for the 0.01 mol/L pH7.4 PBS?5% sucrose and 1% BSA,0.1% Tween20?,gold labeled pad processing liquid and gold standard complex solution were for 0.01 mol/L pH7.4 PBS?7%sucrose,1% BSA,0.4% PVP,1% trehalose?.LS/T6113-2015 grain vomitoxin colloidal gold rapid quantitative detection method provides,the detection limit is 120 ?g/kg.In this experiment,the sensitivity of the test strip DON is 40 ppb.In negative samples of milk and oat add standard detection limit of 40 ?g/L and 70 ?g/kg respectively.Feed detection,soybeans,corn bran,corn grits,buckwheat,rice,etc.Samples.Negative,positive test results and LC-MS/MS test results are approximately the same.The test paper strip has high practical value.