Screening And Optimizing Of Accelerant For High-Producting Lycopene In Neurospora Crassa And The Study Of Antioxidant Activity

Lycopene is one of the carotenoids and natural pigments in plants. It has proved that lycopene has a variety of biological function: antioxidant, scavenging free radicals, anticancer, enhancing immunity, anti-aging, skin protection and so on. That make lycopene be used in medicine, cosmetics and nutritional biochemistry, and many other areas.1. The article explores the measure method microbial of lycopene pigment extract during fermentation, choosing C-18 column to determine the amount of lycopene; The UV detection wavelength is 472 nm; The flow rate is 1.0 mL/min; The column temperature is 28 ?; The inlet sample quantity is 10 ?L; The mobile phase ratio?acetonitrile-methylene chloride, V/V? is 60:40. Lycopene of pigment extract can be detect well. The retention time is 3.110 of lycopene standard solution. The standard curve is y=24.602x-30.721?R2=0.9956?. The recovery is 95% to 105% when adding pigment extract into standard solution. That is proved the experimental results is reliable.2. According to the biosynthesis way of lycopene in microbial cell, added fermentation accelerant during fermentation process. the fermentation accelerant can produce lycopene highly through metabolic regulation. In single factor experiment we make adding time, adding amount as the independent variables, the dry weight, lycopene content of hyphae and spores and lycopene production as the dependent variables, To screen four kinds of significant impact material from citric acid, penicillin, tobacco extract, BHT and TH. Then we optimize four factors?penicillin, tobacco extract, BHT and TH? of influence on lycopene through the response surface method. By the result, the effect order for lycopene production is tobacco extract>TH>penicillin>BHT. The best fermentation accelerant is penicillin1.2 mg/L, tobacco extract 102 mg/L, BHT 3.8 mg/L, TH 1.01 mg/L. Under this condition, the lycopene yield up 13.59 mg/L. In verification tests, lycopene has the highest production?13.50±0.65 mg/L? that closes to forecast. That is proved a potential way for the realization of fermentation production.3. The article extract lycopene from strains using organic?acetone and ethyl acetate? solvents with ultrasonic crushing method. The single factor experiments showed when the lycopene leaching temperature is 65 ?, leaching time is 2.5 h, ratio of solvent is 1:2?ethyl acetate-acetone, V/V?, The lycopene yield is highest. The response surface experiments further confirmed when the leaching temperature is66.66 ?, leaching time is 2.51 h, ratio of solvent?ethyl acetate-acetone, V/V? is 0.64,the yield of lycopene is highest.4.The DPPH radical scavenging ability of the lycopene extract from neurospora crassa, ?-carotene, VC and VE is as follows: ?-carotene > VC > lycopene extract >VE; O₂^-· scavenging ability is as follows: lycopene extract > ?-carotene > VC >VE; ·OH scavenging ability is as follows: ?-carotene > lycopene extract > VC > VE;general oxidation resistance is as follows: ?-carotene > VC > lycopene extract > VE.The lycopene extract from Neurospora crassa has strong scavenging ability and antioxidant capacity. The concentration and antioxidant capacity is proportional.