Extraction And Isolation Of Polyphenols From Gynura Procumbent(LOUR.) MERR And Its Antioxidative And Anti-Inflammatory Studies In

Gynura procumbens(Lour.)Merr.is rich in phenolic compounds,with anti-inflammatory,anti-cancer,anti-virus,antihypertensive,hypoglycemic,lipid-lowering,anti-oxidation,anti-ulcer and other effects.In May 2012,Gynura procumbens was designated as a new source of food by the Ministry of Health.In order to study and promote the use of this vegetable,this paper studied the extraction process,separation and purification of phenolic compounds in Gynura procumbens,The anti-oxidation and anti-inflammatory effects in vitro of Gynura procumbens extract have also been studied.(1)By comparing the content of essential components of different raw materials,the content of phenolic compounds in different raw materials was:water>methanol>ethanol>acetone>ethyl acetate>butanol>trichloromethane,root department>leaf department>stem department.Methanol has toxicity,so we use ethanol and water as the extraction solvent.The optimum extraction method was selected by mixing 50%(volume ratio)ethanol solution with a volume ratio of 1:25(m/V)at 50? and extracting with 21 KHz for 50min.The freeze-dried and 60?hot air drying solution has a stronger antioxidant activity and higher extraction rate of phenolic compounds.(P<0.05).The correlation coefficients of total phenolics,total flavonoids and chlorogenic acid were significantly correlated with total phenolics,total flavonoids and chlorogenic acid(P<0.05),and the correlation coefficients of total phenolics were 0.919,0.848 and 0.907 respectively.The correlation coefficient R was 0.915,0.793 and 0.823 respectively,and the correlation coefficient R with the chlorogenic acid content was 0.887,0.927 and 0.900,respectively.The correlation coefficient with the VC content was low.(2)By separating and purifying the polyphenols in Gynura procumbens polysaccharides,it provides the theoretical basis for the further popularization and application of.In this study,the extraction of crude polyphenols was carried out by macroporous resin column chromatography using ultrasonic assisted extraction of polyphenol extract as raw material.The results showed that NKA-9 macroporous resin was most suitable for the purification of trimethoprim polyphenols.When the NKA-9 macroporous resin was purified,the most suitable conditions were as follows:3.0 mg/mL of the polyphenol extract was loaded at a sample flow rate of 2BV/h.Elution was eluted with 70%ethanol as the eluent and the eluent was 2BV.The purity of trimellitate polyphenols was increased from 24.83%to 78.43%.By HPLC analysis and identification,the separation and purification of peptidoglycerol did not result in the change of phenolic composition and significant loss.Gynura procumbens phenolic substances mainly composed of chlorogenic acid.The antioxidant capacity of DPPH and ABTS free radical scavenging ability was enhanced after purification.(3)To explore the anti-inflammatory and anti-cancer effects of different solvent polarity sites of Gynura procumbens.The LPS-induced RAW 264.7 macrophage inflammation was established by the separation of the polarities from low to high.The extraction of LPS-induced RAW264.7 macrophages was carried out.To investigate the effect of four extracts from Gynura procumbens on the secretion of inflammatory factor-NO.And HPLC-ESI-MS was used to detect the active constituents in the four extracts.The screening of RAW264.7 inflammatory model could significantly inhibit the secretion of NO in model cells and inhibit the proliferation of RAW264.7 cells.Trichloromethane(100,200?g/mL)significantly inhibited the growth of Hela cells.May be related to the gallic acid and terpenoids in the Gynura procumbens.