HPLC Determination Of N,N-dimethyl Formamide In Nosiheptide

Nosiheptide is a kind of ideal feeding antibiotic substitute,with relative safety and good application effect,but N,N-dimethylformamide residuing in the process of refining production involves.After N,N-dimethyl formamide residues invade the body,it will damage the liver and kidney of the human body,it is a moderate toxicity.We must controll N,N-dimethyl formamide residues in the product of nosiheptide,reduce the pollution and hazards of livestock and poultry products.This study looking through waste water,air,CBS N,N-dimethyl formamide residual amount detection method,high performance liquid chromatography detection is established the determination of Nosiheptide in N,N-dimethyl formamide residues.Thro?gh the optimization of chromatographic conditions,the development of detection wavelength was 205 nm,C8 column,20% methanol in water,1.0 m L / min,purified water as the extraction solvent,supermarket extraction three times,for the establishment of Nosiheptide in N,N-dimethyl formamide residues detected by high performance liquid chromatography.By testing the samples,the number of theoretical plates and the separation degree are all in line with the requirements.In this study,the accuracy,the scientific and feasibility of the analysis method are verified,and the analysis method is proved to meet the purpose and requirements of the test.Thro?gh the method validation,this method has a high accuracy.The average recovery rate of more than 95%,precision meet the method validation requirements;excellent in the optimal detection concentration(20mg/ml)25%-350% within the scope of linear;sample detection solution to high temperature and light insensitive,to strong acid,alkali sensitive,the oxidant is very sensitive,but damage resulting from impurities and peak separation of good,does not affect the principal component detection.Detection limit of the method for 2.1?g/mL,limit of quantitation for 8.4?g/mL;good durability,under the conditions of main peak and the adjacent impurity were can effectively separated to tolerance test parameters of small changes,stored for up to 8 hours with good stability.In order to control the residual nosiheptide in the solvent,we promise to the DMF of bacteria peptide,based on methyl formamide liquid chromatography method,thro?gh a large number of detection,statistical analysis of data found that N,N-dimethyl formamide content of nosiheptide were lower than 0.07%(concentration limit a value of 0.088%),meet the requirements of veterinary dr?g residues.Therefore,the DMF residue in the peptide was determined to be no higher than 0.07%,as the release standard of the peptide to ensure the safety of all of the products in the sale of pre-mixed with the premix.The establishment of Nosiheptide N,N-dimethyl formamide residual amount detection method is accurate,reliable,reasonable and feasible,in line with the purpose and requirements of the detection,with great value of popularization and application.