Preparations,Properties And Improvements In Antioxidant Capacity Of Gamma Aminobutyric Acid Enriched Rice Bran

First of all, after the best condition where the fresh rice bran enriched GABA (gamma aminobutyric acid, GABA) was explored, it was suggested that the content of GABA increased by 8 times when the rice bran was cultured about 5 hours under 40? after being adjusted to 30% moisture content by 5 mol/L glutamic acid solution. Besides,different drying methods of rice bran on the stabilization were measured, and at the same time, the content of phenolic acids as well as the antioxidant capacity was investigated. As a result, there was no apparent change of GABA in rice bran cultured under the condition of vacuum drying and freeze drying respectively. Nevertheless, the content of GABA in hot-air drying decreased a lot. And the content of protocatechuic acid, ferulic acid,coumaric acid, sinapic acid, vanillic acid and chlorogenic acid increased to varying degree,among which the increase in ferilic acid was the most obvious. And regarding the comparison of the antioxidant ability between DPPH clearance and ABTS + clearance, the dry processing was beneficial to this kind of ability.Then, after GABA enrich-rice bran was acquired, coupled with a high-fat animal model, the effects of GABA-enrich rice bran and natural rice bran separately on high-fat diet rats, such as blood glucose levels, insulin levels, blood lipid level, oxidative stress, and liver tissue morphology were studied. It was found that the lipid metabolism disorders, liver tissue morphology and function, and body injury caused by oxidative stress of the former were improved a larger degree.Finally, after some genes related to lipid metabolism and oxidative stress in rat liver were detected, including ACC, FAS, Acox1, SREBP-1, Acly1, FAS, GPAM, Dgat1, INSIG1,INSIG2, FATP1, FABP4, were analyzed, it could be concluded that with respect to GABA-enrich rice bran the production of fat and cholesterol in the body were controlled through manipulate gene. Additionally, oxidative stress related genes (GAPDH, PMl, VEGF,HMOXL ) being monitored, it was revealed that the GABA-enrich rice bran was helpful in improving oxidative stress of high-fat diet rats. More importantly, these researches could provide some new ideas in developing and utilizing diverse sorts of rice bran effectively and efficiently.