An Analysis Of The Anti-obesity Effect Of Gama-aminobutyric Enriched Rice Bran

In the study, the obese rats which were induced by high fat diet as the experimental model,and two kinds of rice bran that was ordinary rice bran and GABA enriched rice bran was separately added to the high fat diet in certain proportion, which were dietary intervention. In the feeding period of rats,the experiment mainly detected the change of body weight, blood glucose,mental status,and analysised to lipids,oxidative stress and liver function indicators of the rat serum. which in order to verify the function of this new type of rice bran than ordinary rice bran.And, real-time fluorescent quantitative PCR was used to study the effects of the intervention methods on the expression level of glucose metabolism and lipid metabolism in rats.Firstly, this experiment was prepared rice bran by anaerobic stimulus, namely GABA enriched rice bran. Mealwhile, the properties of this new type of rice bran and rice bran were analyzed. The main contents were the content of gamma amino acid and the antioxidant activity of the two kinds of rice bran. The results showed that the content of gamma amino acid of this new type of rice bran was 8 times than the ordinary rice bran,and the antioxidant capacity was also significantly enhanced.Secondly, the obese rats which were induced by high fat diet as the experimental model,,and two kinds of rice bran that was ordinary rice bran and GABA enriched rice bran was separately added to the high fat diet in 15% proportion,which were dietary intervention. Forty rats were randomly divided into control group and obesity group,were fed with basic diet and high-fat diet,7 weeks of modeling and after modeling success,obesity group was divided for three groups: obese control group, ordinary rice bran group and GABA enriched rice bran group, and the three groups were always fed with high fat dietand were intervented for eight weeks. The weight of obese rats was detected and analyzed:liver function;and other indicators, the observation of liver tissue morphology.The results showed that compared with high-fat group rats, enriched gaba group rats had a certain effect on increasing weight, reducing blood fat. A significant decrease in the liver index and liver damage was observed (P<0.05),as well as an increase in antioxidant capacity (T-AOC) and glutathione (GSH-Px) levels (P<0.05) on GABA enriched rice bran group. GABA enriched rice bran could effectively improve liver injury and oxidative stress level on obesity rats.Finally, the intervention mechanism was analyzed from the level of molecular gene.Through the analysis of the genes,with relating to glucose metabolism, lipid metabolism and oxidative stress in the liver of obese rats, was analyzed the intrinsic regulation mechanism of the rich gamma amino acid to the fat rats. Through the analysis of the liver lipid metabolism related genes mRNA expression changes.The results showed that the functional components of gamma amino acid in rice bran could effectively reduce the synthesis of fatty acids and cholesterol. The further study showed that this intervention enhanced ? oxidation level through the promotion of Acoxl gene expression with the consequence of the improvement of blood lipids.