HPCE Methods For The Determination Of Active Components In Food And Pharmaceutical

In the 21 st century,with the improvement of living standards,the careness of people's fitness has gradually increased.Under the condition of food safety,more people hope that they could adjust their own function by diet.On one hand the need of the basic nutrition could be met,and on the other hand,the disease could be removed.Therefore,the analysis of active components in food and pharmaceutical has attracted much concern currently.Among the many analytical techniques,capillary electrophoresis is widely welcomed by analytical experiments with its advantages,such as higher separation efficiency,shorter analysis time and lower cost consumption.Many new problems have been resolved in different research fields.In this paper,the effective application of high performance capillary electrophoresis-UV absorption detector in food and pharmaceutical analysis were built,the specific contents are as follows:Chapter 1:A brief review of high performance capillary electrophoresis and its application have been introduced,including the development history,the basic separation theory,the separation mode,the injection technology,the signal detection methods and the characteristics of capillary electrophoresis.On this basis,the significance of this paper has been introduced with the application of capillary electrophoresis in various fields in recent years.Chapter 2:A method of high performance capillary zone electrophoresis(HPCZE)was developed for the simultaneous separation and the determination of rutin,kaempferol,isorhamnetin and quercetin in seabuckthorn.The effects of different electrophoresis conditions were investigated.The running buffer were 20 mmol/L Na2B4O7-H3BO3 with 1.5mg/mL beta-cyclodextrin,pH 9.55,the detection wavelength was set at 370 nm,appliedvoltage 25 kV and injection time 5 s.Under these optimal conditions,rutin,kaempferol,isorhamnetin and quercetin could be fully separated from each other within eleven minutes,the linear range were 0.01 to 0.51,0.05 to 0.93,0.02 to 0.65,0.03 to 0.81 mg/m L,respectively,with the correlation coefficient between 0.9971 and 0.9991.The lowest detection limits were5.05×10-5,2.10×10-5,3.75×10-5,1.31×10-5mg/mL,respectively(RSN=3).The average recoveries of spiked samples were in the range from 95.51% to 104.66%,with a relative standard deviation(RSD)lower than 3.92%(n=3).Under the optimized conditions,the mixture of four standard compounds and extracting solution of seabuckthorn produced in three areas were determined.The results showed that the highest content of four compounds of seabuckthorn is from Xinjiang ürümqi,and the lowest content is from Gansu Wuwei.The content of quercetin from Shanxi Lüliang,and that of kaempferol and isorhamnetin from Xinjiang ürümqi are highest.This method is rapid,simple,accurate and reliable for the simultaneous detection of active compounds in seabuckthorn.Chapter 3:A method of high performance capillary electrophoresis(HPCE)was developed for the simultaneous separation and the determination of ploridzine,(-)-epicatechin,rutin and chlorogenic acid in the apple.The effects of different electrophoresis conditions were investigated.The running buffer were 10 mmol/L Na2B4O7-H3BO3,pH 9.2,the detection wavelength was set at 280 nm,applied voltage 25 kV and injection time 5 s.Under these optimal conditions,ploridzine;(-)-epicatechin;rutin and chlorogenic acid could be fully separated from each other within seven minutes,the linear range were 0.005 to 0.120,0.005 to 0.550,0.004 to 0.450,0.020 to 0.600 mg/mL,respectively,with the correlation coefficient between 0.9977 and 0.9992.The lowest detection limits were 3.41×10-5,5.67×10-5,3.78×10-5,3.89×10-5mg/mL,respectively(S/N=3).The relative standard deviations of migration time and peak area were between 0.13% and 4.59%.The average recoveries of spiked samples were in the range from 96.72% to 104.55%,with a relative standard deviation(RSD)lower than 3.74%(n=3).This method is rapid,simple,accurate and reliable for the simultaneous detection of phenolic compounds in apple.Chapter 4 : An efficent high performance capillary electrophoresis method with ultraviolet detector has been developed for the determination of five anthraquinones.Thedetection wavelength was set at 284 nm,separation voltage 25 kV and injection time 5 s.The running buffer were 20 mmol/L Na2B4O7-H3BO3 with 5 mmol/L beta-cyclodextrin,pH 8.50.Under these conditions,emodin,rhein,chrysophanol,physcion and aurantio-obtusin were separated within ten minutes.The linear ranges were 0.004 to 0.350,0.002 to 0.350,0.003 to0.30,0.004 to 0.400,0.005 to 0.400 mg/mL,and the linear correlation coefficient was greater than 0.9952.The limit of detections were 5.03 × 10-5,5.66 × 10-5,1.67 × 10-5,5.19 × 10-5and3.36 × 10-5mg/mL(S/N=3),and the average recoveries(n=3)were 96.29% to 103.64%,with a relative standard deviation(RSD)were 1.39% to 4.56%.The method was fast and accurate,which can provide some feasible reference for the analysis of anthraquinones in cassia.