| Glucose is the main energy source for human body.High blood glucose levels may cause a series of diseases.Therefore,it is of important practical significance to achieve rapid and accurate detection of glucose concentration.So far,glucose sensors have evolved from the original enzyme glucose sensors to non-enzymatic glucose sensors with high sensitivity,low detection limits,and good stability.The sensitive materials of such glucose sensors mainly include metals and their compounds,carbon materials,etc.Among them,the transition metals and their oxides have become the focus of researchers due to their excellent electron transport properties,low price and simple preparation process.Therefore,this paper uses two simple methods to prepare flake-shaped CuO/NiO composites and research on their glucose sensing performance.?1?CuO/NiO composites were prepared by co-precipitation method combined with tubular muffle calcination.The effects of pH,the molar ratio of Cu/Ni and other experimental parameters on the morphology and properties of the composites were investigated.The best synthesis conditions for CuO/NiO composites were:the pH of the reaction solution was 9,and the molar ratio of copper and nickel was 4:1.It was proved that the synthesized material was flake-shaped CuO/NiO by XRD,SEM and other characterization.Glucose sensing performance test was performed on the material-assembled electrodes.The results showed that the sensitivity of glucose detection by CuO/NiO materials synthesized by co-precipitation method was 5 890?A·cm-2·mM-1,the linear range was 4?M-1.6 mM with the detection limit of 0.86?M?S/N=3?.In addition,the CuO/NiO/GCE electrode had good reproducibility all internal electrode and between electrodes?n=5?.It had good anti-interference performance which was not easily affected by interfering substances in the detection process.?2?The hydrothermal method was combined with tubular muffle calcination to prepare flake-shaped CuO/NiO composites.The effects of reaction parameters such as reaction temperature,reaction time and the concentration of precipitation agent urea on the morphology and properties of the materials were investigated.The best synthsized conditions for hydrothermal preparation of CuO/Ni O composites were:the reaction temperature was110?,the reaction time was 24 h,and the urea concentration was 0.2 M.By XRD,SEM and other characterization,it was proved that the synthesized material was flake-shaped CuO/NiO.Glucose sensing performance test was performed on the electrode assembled by CuO/NiO material.The results showed that the sensitivity of glucose detection by CuO/NiO synthesized by hydrothermal method was 3 871?A·cm-2·mM-1,and the linear range was 2?M-2.5 m M,with the detection limit of 0.85?M?S/N=3?.In addition,the CuO/Ni O/GCE electrode had good reproducibility between different electrodes?n=5?and internal electrode.CuO/NiO had good anti-interference performance for it was not easily affected by interfering substances in the detection process.The flake structure may increase the specific surface area of the material,so that the reactive sites increase,and it is goog for electron transport between the electrode and the target,thereby the electrocatalytic oxidation performance of the material to glucose enhances.Human serum samples were tested for CuO/Ni O composites synthesized by two methods,the relative standard deviation of the test results was less than 3%compared with the results of the testing facility,which indicating that CuO/NiO/GCE had certain reliability in practical applications. |
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