| Tropical theileriosis is a tick-borne hemozoon disease characterized by Theileria annulata of Theileriidae Theileria attacks reticuloendothelial system of cells and erythrocytes in bovine. Clinical symptoms are characterized by swollen superficial lymph nodes, fever and anemia of Tropical theileriosis,and acute cases often dead. The disease occurrence and popular are acute in our area such as Turpan and Toksun in which morbidity were more than 60%, caused huge economic losses. Hyalomma detritum is the main spread medium for the disease, so reasons for disease occurs connected with haunted regularity and carrying pathogenic quantity of transmission vector. This study identified Hyalomma detritum on the body surface and feeding environment by morphological observation and molecular biology. And cured Tropical theileriosis cases(N=60) with three groups of drugs in the affected areas, and analyzed curative effect.Provides technical support for preventing and control Tropical theileriosis in this region.(?) In this test, choosed 40 cow farmers to gather medium ticks(N=1088) about cows body surface and feeding environment in Turpan surrounding areas, and morphological identification by ultramicro observation. And designed universal primers, did PCR amplification and sequenced through Hyalomma detritum internal transcribed spacer 2 genes(ITS2), identified by molecular biology. The results showed that 368 ticks were Hyalomma detritum, the dental formula was 3|3, external spur, internal spur were equal length of coxa ?, external spur bended to outer, short pulvillus was about half of claw length, stigmal plate head was oval and premaxilla taper off. Amplified positive bands 518 bp of Hyalomma detritum ITS2 gene by PCR, and sequence similarity was 99% with ITS2 gene of Hyalomma detritum sequences in GenBank(registration number: KC203394.1), consistent with morphological identification consequence. Results indicated that Hyalomma detritum is one of the advantages of Ixodidae in local. It was meaningful for using ITS2 genes to identify hematophagous female ticks(form has changed), nymphal tick and similar Hyalomma on the basis of traditional morphological identification method.(?) This study aimed for curing the clinical Tropical theileriosis cases in epidemic area, and confirmed60 cases through observation of clinical symptoms, etiology examination and PCR of Tropical theileriosis,randomly divided into four groups. And choosed three groups of different drugs for curing, treated group 1(Chinese herbal medicine group), treated group 2(diminazene aceturate group), treated group 3(acriflavine hydrochloride group), and control group. Clinical observation, measurement of temperature, blood smear observation and infestation ratio statistics in 1 d, 2 d, 3 d, 4 d, 5 d and one week for each group separately.The results showed that treated cure rate of group 3 was 93.33%(14/15) higher than group 2 was 86.67%(13/15) and treated group 1 was 73.33%(11/15), treated group 3 and group 1 recurrence rate were 0%(0/15)less than group 2 recurrence rate was 15.38%(2/13). The variance analysis results of different treated group infestation ratio cured patients indicated that control group was very significantly differences(P<0.01) with other groups. Group 2 and group 3 were respectively significant difference(P<0.05) with group 1. Group 2was no significant difference with group 3(P>0.05). The results indicated that treated group 3 was the best in three kinds of prescription groups of curing Tropical theileriosis. At the same time, appropriate using with drugs of Chinese herbal medicine group had a certain therapeutic effect for acute cattle. Above treatment data can lay the foundation for prevention and control of Tropical theileriosis in Turpan. |
PDF Full Text Request