Effects Of Deitary Bioactive Peptides On Growth Performance, Immunity And Antioxidation Of Litopenaeus Vannamei

The study was conducted to investigate the effects of dietary different bioactive peptides sources and levels on growth performance immunity and antioxidation of Litopenaeus vannamei. To find out the optinmal supplemented sources and levels of bioactive peptides. The results would provide the scientific basis for the application of bioactive peptides in Litopenaeus vannamei feed. The results summarized as follows:In trail 1 The effects of bioactive peptides on growth performance, immunity and antioxidation of Litopenaeus vanname were evaluated1050 healthy Litopenaeus vannamei with an average initial body weight of 1.70±0.00 g were randomly stoked into 10 groups with 3 replicates per group and 35 shrimps per tank in recirculated system. Ten experimental diets were prepared in which the two diets with 28% fish meal and 18% fish meal respectively were designated as controls and four bioactive peptides of BAPB, BAPD, BAPR, BAPⅡwith two levels of 1.5% and 3.0% were added into the control diet with the 18% fish meal to make eight test diets. The results indicated that 1) Compare to the group of 18% fish meal, the final body weight(FBW), weight gain rate(WGR), specific growth rate(SGR), feed efficiency(FE) and protein efficiency rate(PER) in the diets with bioactive peptides supplements were significantly higher(P<0.05), other than it had no significant difference on survival rate(SR)(P>0.05). The feed efficiency(FE) and protein efficiency rate(PER) in the diets with BAPB supplements were significantly different with BAPR and BAPⅡ(P<0.05). No significant difference was found in the levels of bioactive peptides(P>0.05). 2) The whole body moisture content in 1.5% BAPⅡwas significantly higher than that in 3.0%BAPB(P<0.05), while no significant difference was found in the other groups. The whole body crude protein content in 1.5% BAPⅡwas significantly higher than that in 3.0%BAPB, 1.5%BAPR, 3.0%BAPR and 3.0%BAPⅡ(P<0.05), while no significant difference was found in the other groups.(P>0.05). The muscle crude lipid content in 18% fish meal was significantly higher than that in other groups(P<0.05), while no significant difference was found in the other groups(P>0.05). There were no significant difference in whole body crude lipid content and muscle moisture and crude protein composition among all groups. 3) The serum alkaline phosphatase(AKP) activity in 1.5% BAPⅡ and 3.0%BAPⅡwere significantly higher than that in other groups(P<0.05), while no significant difference was found in the other groups(P>0.05). The serum lysozeyme(LZM) activity in BAPⅡ was significantly higher than that in other bioactive peptides(P<0.05). There were no significant difference in serum acid phosphatase activity(ACP), AKP activity and LZM activity in the levels of bioactive peptides(P>0.05). The hepatopancreas AKP activity in 1.5%BAPⅡand 3.0%BAPD were significantly higher than that in 1.5%BAPD, 1.5%BAPR and 3.0%BAPR, 18% fish meal and 3.0%BAPⅡwere significantly higher than that in 1.5%BAPD(P<0.05). There were no significant difference in hepatopancreas ACP and LZM among all groups(P>0.05). The serum total antioxidant capacity(T-AOC) in 3.0%BAPⅡwas significantly higher than that in 1.5%BAPB and 3.0%BAPD, while no significant difference was found in the other groups(P>0.05). The hepatopancreas superoxide dismutase(SOD) activity in 3.0%BAPD was significantly higher than that in 1.5%BAPB, 3.0%BAPB and 1.5%BAPD(P<0.05). The hepatopancreas Glutathione peroxidase(GSH-Px) activity in 1.5%BAPD was significantly higher than that in 1.5%BAPB, 3.0%BAPB, 3.0%BAPD and 3.0%BAPR(P<0.05). The hepatopancreas GST activity in 3.0%BAPB was significantly higher than that in 3.0%BAPR, 1.5%BAP and 3.0% BAPⅡ(P<0.05), while no significant difference was found in the other groups(P>0.05). The serum ACP activity, LZM activity, SOD activity, GSH-Px activity, and the hepatopancrea ACP activity, LZM activity, T-AOC, MDA were no significant difference among all groups(P>0.05). The results indicated that dietary bioactive peptides could improve growth, immunity and antioxidation of Litopenaeus vannamei.After the above-mentioned feeding experiment finished, 12 shrimp in esch group were injected with 0.1ml 7.24×105 CFU/ml Vibro Parahaemolyticus. The resulst indicated that 1) compared with 18% fish meal group, the survival rate(SR) of shrimp in 3.0%BAPB was significantly higher than that in 1.5%BAPⅡ(P<0.05), while no significant difference was found in the other groups(P>0.05). There were no significant difference of SR in bioactive peptides sources and levels(P>0.05). 2) The serum LZM activity and hepatopancrea ACP activity in 3.0%BAPⅡwere significantly higher than that in 3.0%BAPB(P<0.05), the while no significant difference was found in the other groups.(P>0.05). The hepatopancrea AKP activity in 1.5% BAPⅡ and 3.0% BAPⅡwere significantly higher than that in 1.5%BAPB(P<0.05), the hepatopancrea LZM activity in 1.5% BAPⅡ was significantly higher than that in 1.05%BAPB, 1.5%BAPR and 3.0%BAPR(P<0.05), while no significant difference was found in the other groups(P>0.05). No significant difference was found in serum ACP, AKP and hepatopancrea AKP among all groups(P>0.05). 3) The serum T-AOC in 1.5% BAPⅡ and 3.0% BAPⅡ were significantly higher than that in other groups(P<0.05), 1.5%BAPR was significantly higher than that in 1.5%BAPB, 3.0%BAPB and 1.5%BAPD, 1.8% fishmeal and 3.0%BAPD were significantly higher than that in 1.5%BAPB and 3.0% BAPB(P<0.05), while no significant difference was found in the other groups(P>0.05). The hepatopancrea SOD in 1.5%BAPD was significantly higher than that in 18% fish meal, 1.5%BAPB, 3.0%BAPB, 3.0%BAPD, 1.5%BAPR and 1.5%BAPⅡ, 3.0%BAPR was significantly higher than that in 18% fish meal, 1.5%BAPB, 3.0%BAPD, 1.5%BAPR and 1.5%BAPⅡ, 3.0% BAPⅡwas significantly higher than that in 1.5%BAPB and 1.5%BAPⅡ(P<0.05), while no significant difference was found in the other groups(P>0.05). No significant difference was found in bioactive peptides sources and levels(P>0.05). The hepatopancrea GSH-Px in 1.5%BAPD was significantly higher than that in 18% fish meal, 1.5%BAPB, 3.0%BAPB, 3.0%BAPD, 1.5%BAPR and 1.5%BAPⅡ, 3.0%BAPB and 1.5%BAPR were significantly higher than that in 1.5%BAPB(P<0.05), while no significant difference was found in the other groups(P>0.05). There were no significant difference of hepatopancrea SOD, T-AOC, GSH-Px and GST in bioactive peptides sources and levels.(P>0.05). There were no significant difference in serum SOD, GSH-Px and hepatopancrea T-AOC, GST among all groups(P>0.05). The In trail 2 Vibro Parahaemolyticus resistance of Litopenaus vannmei results indicated that the supplement of bioactive peptides could increase the Vibro Parahaemolyticus resistance of Litopenaus vannmei, improve the immunity, antioxidation performance of Litopenaus vannmei, and the optimum source was 1.5%.BAP Ⅱ.