Effects Of Hydrolysates From Soybean And Gluten With Pepsin On Immunity In Rats

Part I Preparation of soybean and gluten hydrolytic peptidessoybean and gluten were prepared with the method of alkali extraction and acid precipitation and sodium chloride buffer washing respectively. Both of them were hydrolyzed at 37℃, pH1.4 for 2hs with pepsin at the ratio 30 : 1 between substance and enzyme. Then the degree of hydrolysis ( DH ) was measured with the method of triketohydrindene hydrate. The results showed that the nitrogen concentration of soybean and gluten protein in hydrolyzate were 2.15 ±0.04 mg/mL and 1.20±0.00 mg/mL respectively (n=2). The DH of soybean and gluten hydrolysates were 14.49% and 12.99%, respectively. The average length of peptides of two hydrolysates were 6.9 and 7.7 . Part II Effects of feeding hydrolysates from soydean and gluten with pepsin on body weight in rats100 rats ( 28days old ) were divided into 4 groups randomly, every group including 15 male rats and 10 female rats. The four groups had been fed soybean (control I group, con I), gluten (control II group, con II), soybean hydrolysates (experiment I group, exp I) and gluten hydrolysates (experiment II group, exp II) respectively, for 21 days successively. On the base of general diet the feeding dose is 1 mL hydrolyzate, and the concentration of protein is 5 mg/ mL. The rats was weighed on the 35th day and the 49th day respectively. With univariate analysis, the results showed there was no significant difference among 4 groups on 35th days. Compared with that of con I, weight of exp I was increased by 10%. Compared with that of con II, exp II was raised by 9.3% than that of con II. Both of them had no significant variation. But on the 49th day there existed greatly significant difference between exp II and con I (p<0.05).Part III Effects of hydrolysates from soydean and gluten with pepsin on immunity in ratsSeries I Effects of hydrolysates from two kinds of protein on weight of immune viscera in rats4 groups rats from Part II were killed for analyzing weight changes of thymus and spleen at the end of Part II experiment. The results showed that the thymus and spleen weight of exp I were 40% (p<0.01) and 29.7% (p<0.01) higher than those of con I respectively, and those in exp II increased 37.1% and 41.0% (p<0.01) than those in conll . Meanwhile , analysis results of thymus and spleen relative weight are similar to those of absolute weight of immune viscera.Series II Effects of hydrolysates from two kinds of protein on phagocytosis ability of celiac macrophagus in rats4 groups of rats from Part II were killed and harvested celiac macrophages aseptically.Phagocytosis of celiac macrophages test (MTT) was performed. The results of the MTT showed that soybean hydrolysates can enhance the phagocytosis significantly . The As7o value increased 67% (p<0.01) than in con I. Compared with that of con II, the A570 value of exp II was increased by 12% , but no significant difference. The A570 in exp I was 42.5% (p<0.05) higher than in exp II. The results showed that hydrolysates from two kinds of protein can improve phagocytosis of celiac macrophagus, but activity of them exists in difference.Series III Effects of hydrolysates from two kinds of protein on lymphocytic transformation in ratsBlood lymphocytes were sampled from rats treated by Part II experiment aseptically. Lymphocytic transformation test (LTT) was performed by 3H-TdR method. The results showed that the stimulated index (SI) of exp I was greatly higher than that of con I (p<0.01). Compared with that of con II, SI of exp II was improved by 16.2%. but the difference was not significant. Through multi-compared analysis, the SI in exp I increased by 12.2% than in exp II with no significant difference, and that in con II was 23% higher than con I with no significant difference. The results showed that hydrolysates from two kinds of protein can accelerate lymphocytic transformation, and soybean peptides are better than gluten peptides .Series IV Effects of hydrolysates from two kinds of protein on HI titer in serum in rats...