The Establishment Of Rapid Propagation System Of Moringa Oleifera Lam.

Moringa oleifera Lam. belongs to Moringaceae family which is a valuable species with multiple functions. The goals of this study are to establish the tissue culture and rapid propagation system of Moringa oleifera LAM, The explants were taken from different parts of the seedlings, and methods of multiple-shoots proliferation and organogenesis, were applied to compare the regeneration ability of those explants taken from different parts of seedling. The results showed as bellows:1. It can effectively reduce the pollution rate and improve the germination rate up to 87%, when the explants removed seed coat with 1‰ Hg Cl2 15 min sterilization.2. The best medium to induce callus from the leaves of Moringa seeds was: MS+6-BA1.6 mg/L+NAA0.05 mg/L, and the inducing rate was up to 82.5%.3. The best medium of increasing bud number from the top bud of Moringa seeds was: MS+6-BA0.4 mg/L+IBA0.05 mg/L, and the multiplication rate can be up to 2.13.4. The best medium to induce callus from the epicotyl of Moringa seeds was: MS+6-BA1.2 mg/L+NAA0.05 mg/L, and the inducing rate was up to 76.67%. Dark environment treatment can effectively shorten the time of callus formation and improve the induce rate.5. The best medium to induce clump buds from the cotyledon of Moringa seeds was: MS+6-BA1.2 mg/L+NAA0.05 mg/L, and the multiplication rate can be up to 6. Budding time of the cotyledon of Moringa without hypocotyl was earlier than those with hypocotyls and showed more multiple buds, leaves and sturdy stems.6. The best medium to induce callus from the hypocotyl of Moringa seeds was to place the tissue in the light scattering in MS+6-BA1.6mg/L+NAA0.05 mg/L medium, and the inducing rate was up to 86.67%. A few buds emerged and elongated from callus.7. The best medium to induce callus from the stem of Moringa seeds was: MS+6-BA1.2 mg/L+NAA0.1 mg/L, and the inducing rate was up to 83.33%.8. The best medium to induce adventitious bud from the stem nodal of Moringa seeds was: MS+6-BA1.6mg/L+NAA0.1 mg/L.9. The ideal test for light intensity of the stem nodal was that the explants culture under dark environment, when a bud points appeared and then continue to develop into normal illumination. Five internodes should be placed in one bottle if the explants with more adventious bud were chosen. If chose the bud height of explants induction, choose a bottle placed three internodes. The regeneration ability of the stem nodal was influenced by genotype. The suitable culture vessel to induce adventitious bud from the stem nodal was plastic bottle with a breather hole on lids.10. The best medium to induce root from the Moringa seeds was: MS+NAA0.1 mg/L, and the average rooting volume was up to 10.8.