The Transcriptome Data Assemebly Of Several Tissues Of Moringa Oleifera Lam. And The Reannotations Of Moringa Oleifera Lam. Genome

Moringa oleifera Lam. is indigenous to north-western India, the tree is characterized as a fast-growing, drought-tolerant type. Since it contains abundant nutrients, it is considered as a kind of ideal food. Moringa has long been recognized in traditional medicine worldwide as it can be a preventative and treatment agent of several health conditions, including treatments for inflammation, infectious diseases, cardiovascular diseases, gastrointestinal,haematological and hepatorenal disorders etc. and it is also a natural antioxidant and an antitumor promoter. Obviously, Moringa oleifera Lam. is an important agricultural plant with significant values. Therefore, Moringa oleifera Lam. is widely studied and accepted in China in recent years.However, little basic research on M. Oleifera has been conducted, which greatly limits its further applications. So, in 2014, a reserch team reported a high-quality draft genome sequence of M. oleifera at 289 Mb. This assembly represents 91.78% of the estimated genome size and contains 19,465protein-coding genes. With this reference genome, we can extend our understanding toward M. Oleifera by transcriptome approach.Therefore, it is necessary to carried out high-throughput transcriptome sequencing to generate enormous transcript sequences for further understanding of gene expression in M. Oleifera and provide transcriptomic information for reannotation of the genome and other further research. In this study, we collected12 tissues in different growth periods of M. Oleifera, including the root, stem,leaf, flower, seed etc. Then we sequenced their RNA with illumina paired-end sequencing technology. By assembling transcriptome sequencing data, we reannotated the M. Oleifera genome, and computed the abundance of gene expression in these tissues of M. Oleifera. Furthermore, all the assembled unigenes were searched against the NCBI non-redundant(nr), Swiss-prot, KEGG and COG databases.In this study, more than 120 G transcriptome data was obtained. So, the 12 tissues of M. Oleifera’s transcritomic sequences are accessible now. With these data, we renewed the genome annotation of M. Oleifera. Also, we gainedstatistical data of the abundance of gene expression in 12 tissues of M. Oleifera.Furthermore, the similarity search indicated that more than 4,000 unigenes showed significant similarities to known proteins from major databases. This research generated a substantial transcriptome sequences of M. Oleifera, which will be very useful resources for gene annotation and related research.