Effects Of Lacti-glyceride On Anti-oxidative Function, Energy Metabolism Status And Intestinal Mucosal Morphological Struct In Broilers In Low Temperature Environment

This Study was conducted to investigate the effects of lacti-glyceride on anti-oxidative function,energy metabolism status and intestinal mucosal morphological struct in broilers in low temperature environment.150 1-day-old Cobb chickens were randomly divided into three groups(control group,low temperature group,lacti-glyceride group).Each treatment has 5 replicate pens and 10 chickens per pen.The control group and low temperature group were fed a basal diet,whereas lacti-glyceridegroup were fed basal diet + 500 mg/kg lacti-glyceride.The low temperature process was followed as:first week 32?34 ?,second week 30?32?,third to sixth week 26 ± 2?(the control group)and 10 ±2 ?(the other groups).The humidity of all groups 50%?60%.The results showed that:1.The effects of lacti-glyceride on growth performance,blood biochemical parameters,the relative weight of organ and intestinal mucosal morphological in broilers in low temperature environment.(1)Compared with the control group,the low temperature treatment decreased the average daily gain(P<0.05)(15-42d and 1-42d),but increased the ratio of feed to gain(P<0.05)(15-42d and 1-42d).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride increased the average daily feed intake(P<0.05)(15-42d and 1-42d),but had no influences on the average daily gain and the ratio of feed to gain.(2)Compared with the control group,the low temperature treatment increased the ALP activity and the level of TG in the blood(P<0.05),but decreased the ALT activity(P<0.05).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride decreased the ALT and ALT activities(P<0.05),and the levels of TG and TBIL(P<0.05).(3)Compared with the control group,the low temperature treatment decreased the weight of the thymus,bursa of fabricius and leg muscle(P<0.05),but increased th weight of the heart(P<0.05).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride increased the weight of the leg muscle(P<0.05).(2)Compared with the control group,the low temperature treatment decreased the villus height and the ratio of villus height to crypt deep in the duodenum(P<0.05),the ratio of villus height to crypt deep in the jejunum(P<0.05),and the villus height and crypt deep in the ilenum(P<0.05),but increased the crypt deep in the jejunum(P<0.05).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride increased the villus height and the ratio of villus height to crypt deep in the duodenum,jejunum and ilenum(P<0.05),decreased the crypt deep in the duodenum,jejunum and ilenum(P<0.05).2.The effects of lacti-glyceride on anti-oxidative capity of tissue in broilers in low temperature environment.(1)Compared with the control group,the low temperature treatment decreased the SOD and CAT activity in the blood of broilers(P<0.05),as well as the SOD,CAT and GSH-Px activity in the heart(P<0.05),the SOD activity in the liver(P<0.05),the SOD,GSH-Px activity in the lung(P<0.05),the CAT activity in the duodenum(P<0.05),the SOD,CAT and GSH-Px activity in the jejunum(P<0.05),but increased the GSH-Px activity in the duodenum(P<0.05).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride increased the SOD and GSH-Px activity in the blood(P<0.05),the GSH-Px activity in the heart(P<0.05),the SOD activity in the liver(P<0.05),the CAT activity in the lung(P<0.05),the SOD and CAT activity in the duodenum(P<0.05),the SOD and GSH-Px activity in the jejunum(P<0.05).the SOD activity in the ilenum(P<0.05),but decreased the CAT in the blood(P<0.05).(2)Compared with the control group,the low temperature treatment increased the HIF-1 mRNA level in the duodenum(P<0.05),but increased the HMOX-1 mRNA level in the jejunum(P<0.05).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride increased the HMOX-1 mRNA level in the duodenum(P<0.05),the HMOX-1 and HIF-1 mRNA levels in the jejunum(P<0.05),bu decreased the HIF-1mRNA levels in the duodenum and ilenum(P<0.05).3.The effects of lacti-glyceride on heart,liver and intestinal energy metabolism status in broilers in low temperature environment.(1)Compared with the control group,the low temperature treatment decreased the levels of ATP and TAN in the heart,the level of ATP in the liver(P<0.05),the levels of ATP,TAN and EC in the duodenum(P<0.05),the levels of ATP,ADP and EC in the ilenum(P<0.05),but indcreased the level of ADP and the ratio of AMP/ATP in the liver(P<0.05),the ratios of AMP/ATP in the duodenum and jejunum in broilers under a low temperature environment(P<0.05).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride increased the level of ATP and EC in the heart,duodenum and ilenum(P<0.05).(2)Compared with the control group,the low temperature treatment increased the AMPK-al mRNA levels in the heart,duodenum,jejunum and ilenum(P<0.05).In comparison with the low temperature group,diet supplemented with 500 mg/kg lacti-glyceride decreased the AMPK-?1 mRNA levels in the heart,duodenum,jejunum and ilenum in broilers in low temperature environment(P<0.05).In conclusion,the lowtemperature treatment decreased the growth performance,anti-oxidative capity,energy metabolism status and intestinal mucosal morphological struct in broilers.Diet supplemented with 500 mg/kg lacti-glyceride could improve anti-oxidative capity,energy metabolism status and intestinal mucosal morphological struct in broilers in low temperature environment.