Study On Pollen Viability Of Potentila Reptans

Pollen is regarded as an important part of plant genetic material. In plant genetic and germplasm resources preservation and so on, it plays a particularly important role. This test has used Potentilla Reptans pollen for materials. First of all, the experiment is carried out around the pollen vitality determination, by determining the best measurement method, the optimal pollen collection time, the best medium components, the different germination and flowering temperature impact on pollen vitality. Getting the strongest vitality of Potentilla Reptans pollen, is made preparation for storage experiment. Then, pollen of drying process was studied. Effects of various preservation temperature and time on the Potentilla reptans pollen viability were studied. Specific results are as follows:1. Potentilla reptans pollen flowering period could be divided into early flowering, pollen collection period of full bloom, flowering late, then the strongest pollen vitality of the full bloom period. Studying of pollen day life vitality, early 9:00 pollen germination rate is the best.2. In order to determine the most suitable for the determination of pollen germination rate method, to the following process:TTC method, I2-KI staining method and off-body culture method. For staining degree of staining method without a clearly defined, so the error is oversize. Staining method is not suitable for the detection of Potentilla reptans pollen viability. The results of the liquid culture method showed that pollen broken and inclusions were overflowed. So the solid culture medium can be the first choice of testing pollen viability.3. In order to study the effects of different culture media on pollen viability of Potentilla reptans, to 10g/L agar acid as the basic, took the pollen germination must nutrients of sucrose, and the promotion of sucrose absorption boric acid as medium optimization of the two factors, the design of five levels of combinatorial optimization. The results showed that 100g/L sucrose acid and 100mg/L boric were the optimal conditions for Potentilla reptans pollen germination, in which the rate of pollen germination was 52.31%. GA3 and more than 40mg/L concentration of CaCl2 can inhibit pollen germination, but the 40mg/L suitable concentration of CaCl2 promote pollen germination, the rate of pollen germination was 54.00%.4. The experiment that the influence of germination temperature to Potentilla reptans pollen germination rate design 5 germination temperature level6?,12?,18?,25? and 30?. The best pollen germination rates was 52.31% in 25?. As the debasing of germination temperature, the pollen viability going down obviously. The pollen germination rates were mensurated in the different florescence and germination temperatures. The interaction of florescence temperature and germination temperature is high significant, the effect of germination temperature to pollen germination rate was bigger than florescence temperature.5. This experiment was to Potentilla reptans pollen silica gel, natural rose, oven drying. So silica-gel dring derived was the best storage conditions and the pollen viability was 55.69%. In 4? low temperature, 20?room temperature,35? high temperature three different temperatures, to the pollen for dry and no dry processing. The extension of storage time and preservation temperature, the pollen viability came down. It was more contribute to maintaining pollen viability at low temperature with drying condition.