Bioinformatics Analysis Of HD-Zip Gene Family And Identification Of HD-Zip ?Genes Related To Stress Resistance In Tomato

With the continuous expansion of tomato planting,high salt,drought,low temperature,pathogen and other non-biological and biological stress on tomato growth and production caused serious harm.So far,a large number of studies have shown that,the plant transcription factor in plant adaptation and resist all kinds of creatures and in the process of biological stress plays an important role in regulating.For different plant transcription factor family research in recent years more and more deepper,HD-Zip is peculiar to plant transcription factor,involved in plant a variety of biological functions,especially for plants under abiotic stress response has an important regulatory role.In this study,we analysis of HD-Zip transcription factor by bioinformatics,include physicochemical properties,conserved,gene structure,chromosome distribution,secondary structure and third-dimensional structure.At the same time,we analyzed the expression pattern of tissue specificity and abiotic stress in tomato HD-Zip I subfamily,and constructed the VIGS system.The research aimed to provide the basis for the study on the function of tomato HD-Zip gene family in abiotic stress responset.The main results are as follows:Tomato HD-Zip transcription factor was identified to contain 58 members(Sl HZ1-Sl HZ58),which were divided into Sl HD-Zip I-IV subfamily,all 58 genes are not evenly distributed on the12 chromosomes.The amplification of HD-Zip genes in tomato was mainly fragment replication.The secondary structure of the member is mainly helix and similarity between third-dimensional.15 Sl HD-Zip I transcription factors were expressed at different levels in roots,stems,leaves,flowers and fruits.Most of the genes are expressed in roots,while the Sl HZ30 gene is expressed only in flowers.Adversity stress expression analysis,under salt stress treatment the expression of 14 genes were up-regulated and 1(SlHZ14)gene down-regulated.In the drought treatment,the expression of13 genes were up-regulated and 2(Sl HZ14 and Sl HZ28)genes down-regulate.In low temperature all 15 genes up-regulated,including Sl HZ13 raised the most significant genes.Quantity change the expression of adversity stress response analysis of significant genes SlHZ11 and Sl HZ15 VIGS analysis,gene silencing found Sl HZ11 and SlHZ15 gene silence,plants under salt and drought stress conditions considerably increase the index of MDA,shows that thelower gene expression can reduce the resistance to salt and drought resistance of plant.