Efficient Regeneration System Of Hydrangea Macrophylla Leaf Was Established And Effect Of Dark Culture On Enzyme Variance During Regeneration

In order to establish a high frequency regeneration system for Hydrangea macrophylla Leaf, the crucial effect of plant growth regulators, the raw part of blade and blade cutting way, sucrose concentration, the basic culture medium, AgN03, light quality, processing time, genotype and PH value on plant regeneration in H. macrophylla 'Adria' were investigated using leaf as explant.Explore the PH of H. macrophylla.'Adri' leaf regeneration and the influence of matrix of best hydrangeas take root and leaf physiological indexes change under dark treatment.1. appropriate medium Using the sterile tissue culture leaves of H.macrophylla 'Adria' as explant, Types of culture medium, hormone type and ratio of hormones on bud differentiation effects, The result showed that option bud differentiation medium was MS+TDZ3.0mg/L+NAA0.3mg/L, and bud differentiation rate was 36%,2. The influencing factors of hydrangea leaf regeneration The result showed that the top leaf a is the best experiment material.The option choice which could bud differentiation of the leaves was darkness treatment for 15 days, and the best pH is the 5.6 and bud differentiation rate was 98%. Buds over 3cm height could from adventitious roots in 1/2MS supplemented without hormone, and the rate of rooting was up to 100%.3. Physiological indexes of leaves under dark treatment Using the sterile tissue culture leaves of H. macrophylla 'Adria' as explant. In the control, the leaves were treated by T0, and the leaves were cultured under the dark condition of 5,10,20,15 and 25d, respectively, and recorded as T1, T3, T2, T4 and T5 respectively. The relationship between regeneration frequency and POD activity,SOD activity CAT activity, MDA activity, soluble protein content and soluble surger was studied by H.macrophylla'Adri'Leaf.The results showed that the dark cultivation could not only contribute to POD activity grow, but also conduce to its activity remained at a high level. The SOD activity and soluble protein content could show the changes of leaf physio logical state, but could not reflect the effect o f dark cultivation on the regeneration frequency. In summary, dark treatment can help to incr ease POD activity and remain it at a high level.With the increase of dark incubation time, hydrangea leaf MDA content change is smaller, the activity can be reduced to about 10 mol/g;The content of soluble protein change is stable, but the experimental group volatile content of soluble protein is the longer the change.But the blade of dark train 15 d at the peak have a value of maximum,and the value can reach more than 30 mol/g.The change of soluble sugar content than the control group, There are two times the most resources.after 30 days earlier, the experimental group is a significant trend of high content of soluble sugar. CAT and soluble surger activity as indicators can explain the dark cultivation for cultivation effectiveness.