A Study On Tissue Culture For Hydrangea Macrophylla

Hydrangea macrophylla is native to our country and Japan, and has the high ornamental value and the economic value, so it was chosen as the key bush in 2008 Beijing Olympic Games city afforestation. Presently, Hydrangea macrophylla are mainly bred through sowing seeds and the cutting, but these ways have difficulty to satisfy the production and the application need. Stems segment and leaves of Hydrangea macrophylla were used as source of initial explants in this study. We studied on the effect of factors which affected the tissue culture and axillary bud, such as selected, pre-sterilization, the bud induction, callus induction, rooting and also studied the factor affecting plant growth regulator such as illumination condition, sucrose and other factors. And then set up a regeneration system of Hydrangea macrophylla by using the technology of tissue culture for factory production, the mains results are as follows:(1)The best method for sterilize: explants were dip in 70% ethyl alcohol for 30s, then agitating in a solution of 0.1%HgCl2 for 10 min for stem, and finally rinsed five times with sterile distilled water. The most appropriate hormone combination for axillary bud germination was MS + 6-BA0.5mg/L+IBA0.5mg/L; the optimum hormones combination for the proliferation of axillary bud was MS + 6-BA3.0mg/L +IBA 0.1 mg/L; the best hormone ratio for rooting: 1/2 MS + IBA0.3mg/L. Illumination was better than darkness for the callus induction and the bud growth. And the most appropriate illumination was the natural light with 1500lux-2000lux. The rooting rate attained 100%. Meanwhile, the quality of the root system was better than others. The survive ratio could reach 100% when they were transplant to the medium composed with one part pearlite, two part vermiculite.(2)No vaccine leaves which were received in cultivation of stem as the experiment materials were used in callus induction test and plantlet regeneration test. The best hormone ratio was MS + 6-BA1.0mg/L + IBA1.0mg/L. the induction rate of callus was 79.46%. The best medium for the proliferation: 1/4MS +6-BA0.5mg/L + IBA0.2mg/L, the proliferation coefficient was 9.2; the best medium for the differentiation: MS +TDZ0.1mg/L + IBA0.5mg/L, the differentiation coefficient was 48%.