Studies On The Chemical Constituents From Chimonanthus Praecox Flower And Its Extractions Obtained By Supercritical CO₂

The cultivation of Chimonanthus praecox has a long history in our country,rich in resources,but at present,there are not many reports about the main chemical compositions,essential oil extraction and bioactive constituents of the Chimonanthus praecox flower.In this paper,according to the research methods of plants,the material composition of the Chimonanthus praecox flower is studied systematically,comparison of the Chemical Constituents among the four common varieties of Chimonanthus praecox flower is made and the main products of supercritical CO₂ fluid extraction is studied.This paper aims to provide some theoretical basis for the development and utilization of the products such as Chimonanthus praecox flower and its essential oils.The main results of this paper are as follows:（1）It is found that the Chimonanthus praecox flower contains amino acids,peptides,vitamins,steroids,coumarin,triterpenes,volatile oils,oils and other substances,the basic non-biological alkali,cardiac glycosides and other substances through the system pre-test method.（2）The content of the substance in the Chimonanthus praecox flower: water-soluble substances> wlcohol-soluble substances> liposoluble substances;Four kinds of Chimonanthus praecox flower sample effective extraction of material content: Suxin> Nanjing qingkou> Gouya > Jinhong qingkou;The content of the main components in the four kinds of Chimonanthus praecox flower is small,the content of protein content is highest in substance content,it is between 241.85 and 272.24 mg/g.（3）The volatile substances in four kinds of Chimonanthus praecox flowers are analyzed by Gas Chromatography-Mass Spectrometer（GC-MS）using Solid-Phase Microextraction（SPME）.A total of 121 compounds are identified,including 57 olefins,5 aldehydes,11 alkanes,18 alcohols,16 esters,4 ketones and 14 other substances.The volatile substances mainly contains olefins,alcohols and esters,and the content of olefins is highest.（4）To some extent,the content of the main components of the Chimonanthus praecox flower are decreased after supercritical CO₂ extraction,but it still have a lot of effective substances.Decrease of substance extraction rate: alcohol-soluble substances>water-soluble substances> fat-soluble substances;in addition to ash and crude fiber content has increased,the other basic components are decreased,descending ratio: amino acid> soluble total sugar> protein content> reducing sugar> total flavonoids> crude fat> polysaccharide.（5）We can get three essential oils through separation and concentration.The results of their contents with the GC-MS analysis: 69 kinds of alcohols,48 kinds of terpenes,32 kinds of esters,28 kinds of alkanes,25 kinds of ketones,21 kinds of aldehydes,43 kinds of other substances is fond in three essential oils.The results of sensory evaluation: water-soluble essential oil get highest score on the appearance;Fat-soluble liquid essential oil get highest score on fragrance.Total score: fat-soluble liquid essential oil> fat-soluble solid oil> water-soluble essential oil.（6）The chromatographic conditions of High Performance Liquid Chromatography about three kinds of internal standard are established by single factor experiment: A、nonylaldehyde: detection wavelength: 288 nm;injection volume: 40 uL;mobile phase: 90% acetonitrile;total flow rate: 0.7 m L/min;column temperature: 40 °C;B、linalool: detection wavelength: 212 nm;injection volume: 3 uL;mobile phase: 40% acetonitrile;total flow rate: 1.0 mL/min;column temperature: 45 °C;C、（-）-oxidized carnosine: detection wavelength: 214 nm;injection volume: 10 u L;mobile phase: 75% acetonitrile;total flow rate: 0.9 m L/min;column temperature: 30 °C;The regression equation what include the peak area（X）and the peak area（Y）at the corresponding concentration,and minimum detection limit about the three kinds of internal standard: nonanal: Y = 299823 X + 8672.4,0.004;linalool: Y = 107 X + 17119,0.002;（-）-oxidized carnosine: Y = 107 X + 85532,0.002;Nonylaldehyde,linalool and（-）-oxidized carnosine in fat-soluble liquid essential oil are determined to be 0.0756 ± 0.0011 mg/m L,0.0574 ± 0.0005 mg/mL and 0.0451 ± 0.0006 mg/m L,respectively.