The Study Of Relationship Between ERK1/2 And P38 MAPK Signal Pathway And LOX Expression In Gastric Cancer Cells

Objective To explore the corelations of the expression of lysyl oxidase(LOX) and p38 MAPK or ERK1/2 MAPK signal pathway in gastric cancer cells, and evaluate the regulatory mechanism of LOX, and try to provide the experimental data about the effects of LOX in the invasion metastasis.Methods Gastric cancer cell line MGC-803 and BGC-823 were cultured in vitro. After exposing the cells to different concentration of SB203580 or U0126, which inhibited p38 MAPK or ERK1/2 respectively, the proliferation of cells was detected by MTT colorimetric experiment; the migration of MGC-803 cells was assessed by wound-healing assay; the relative expression levels of LOX was detected through western-blot and immunofluorescence assay. The expression levels of p-p38 MAPK, p38 MAPK, p-ERK1/2 and ERK1/2 were measured using Cell-Based EGFR(Active) ELISA when LOX was inhibited by ?-Aminopropionitrile(BAPN).Results 1?The cell proliferation curves were drew through MTT assay. Compared with untreated group, the amounts of MGC-803 and BGC-823 are similar after treated with 5?M, 10?M and 20?M SB203580, 4h(p<0.05). The number of survival MGC-803 cells reduced significantly after treated with 5?M, 8h; The number of survival MGC-803 and BGC-823 cells decreased significantly after treated 16 h, 24 h and 48 h with 5?M, 10?M and 20?M SB203580(p<0.05). The number of survival MGC-803 and BGC-823 cells reduced significantly after treated 24 h, 32 h and 48 h, when treated with 5?M, 10?M and 20?M U0126(p<0.05).2 ? The results of wound-healing assay showed, the abilities of MGC-803 migration were inhibited by treated cells with 5?M SB203580 or U0126 36h(p<0.05), and treated cells with 10?M SB203580 or U0126 12 h, 24 h and 36h(p<0.05).3?The results of western-blot and immunofluorescence assay illustrated that the expression of LOX decreased after treated the cells using 5?M and 10?M SB203580 or U0126 compared with untreated group.4?When LOX was inhibited by 0.1m M and 0.2m M BAPN, the abundances of p38 MAPK, p-p38 MAPK in MGC-803 and BGC-823 are similar as untreated cells. But after LOX was inhibited by 0.3m M BAPN, the level of p-p38 MAPK increased. In the meanwhile, the expression of p-ERK1/2 and ERK1/2 are similar in different groups(p>0.05).Conclusions The abilities of cell proliferation and migration can be affected through p38 MAPK and ERK1/2 MAPK signal pathway. The expression of LOX also can be adjusted through p38 MAPK and ERK1/2 signal pathway probably. But the ERK1/2 MAPK signal pathway can not be affected by LOX.