| Skin plays the role as the first immune barrier protecting the body from outside environment. Being subjected to constant UV irradiation would cause the accumulation of free radical and peroxide in the skin cells. The body’s self-defense mechanisms would also be weaken, introducing photo-damage of the skin, pigmentation and even the formation of melanin tumor. In recent years, the positive role of Lactic Acid Bacteria on healthy development has been widely organized. Its antioxidant activity has also been proved in some research studies. However, few studies have focused on the influence of Lactic Acid Bacteria or its fermentation products on the ultraviolet (UV) irradiation-induced skin aging and photo-damage. The aim of our study is to evaluate the effects of NS-FS on skin’s antioxidant status and its relating molecular mechanisms. Our work have involved both in vivo and vitro. In vitro, through the ABST+ and DPPH experiments, we find that NS-FS exhibit strong radical scavenging activity. In addition, NS-FS can significantly inhibit the formation of Sin-1-introduced ROS in HaCat cells, indicating the effective antioxidant status of NS-FS. Then we further examined its antioxidant status in vivo experiment by building a module, using hairless mice(SKH-1) under ultraviolet B (UVB) irradiation. The results show that NS-FS can effectively relieve the skin’s photo-aging of hairless mice by reducing the thickness of the epidermis and transdermal water loss (TEWL). Besides, NS-FS can effectively ameliorate the activity of anti-oxidation enzyme(including CAT, GPx) and its expression level. We also find that NS-FS can improve the activity of Nrf2, introducing antioxidant status of skin cells, and as a consequence, protecting the skin from photo-aging. Taken together, our study have examined the effects of NS-FS on skin’s antioxidant status and its relating molecular mechanisms, providing experimental foundation for the development of Probiotics-relating skincare cosmetics. |
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