Effects Of Fermented Camel Milk Prepared In Laboratory On Diabetic Rats

Objective:To observe the effects of fermented camel milk（shubat）containing ten lactic acid bacteria and four yeasts on diabetic rats and to study the preliminary mechanism.Methods:Diabetic rats were induced by an administration high-glucose-fat diet for 8weeks and an intraperitoneal injection of streptozotocin(STZ,30 mg·kg^-1).Diabetic rats were divided into four groups and treated for 4 weeks with sitagliptin(30 mg·kg^-1)or shubat（6.97×10⁶ lactic acid bacteria+2.20×10⁴ yeasts）CFU/mL/kg,（6.97×10⁷ lactic acid bacteria+2.20×10⁵ yeasts）CFU/mL/kg and（6.97×10⁸ lactic acid bacteria+2.20×10⁶ yeasts）CFU/mL/kg.In addition,a normal control group and a diabetic control group were used for comparison.1.Fasting blood glucose（FBG）and body weight（BW）were measured before treatment and every week thereafter.Glucose（Glu）,glucagon-like peptide-1（GLP-1）,glycated hemoglobin（HbAlc）,C-peptide（CP）,Total cholesterol（TC）,triglycerides（TG）,low-density lipoprotein cholesterol（LDL-c）,high density lipoprotein cholesterol（HDL-c）,Serum creatinine（SCr）,blood urea nitrogen（BUN）,alanine transaminase（ALT）,aspartate transaminase（AST）and pancreas tissue sections were tested after 4 weeks.Results:High dose fermented camel milk decreased FBG and HbAlc,increased C-peptide（P<0.05）and GLP-1（P<0.01）levels,decreased serum TC,TG and LDL-c（P<0.05）,increased HDL-c（P<0.01）levels,improved the reduction of body weight（P<0.01）and the liver and kidney function compared to diabetic controls.Histological analysis showed fermented camel milk protected the function of islets of diabetic rats.Conclusion:The results of this study indicates that fermented camel milk decreased FBG and HbAlc,increased C-peptide and GLP-1,modulated lipid metabolism and protected liver and kidney injury in type 2 diabetic rats,which may be related to various probiotics acting through promoting the release of GLP-1 and protecting the function of islets.