The Expression And The Relationship Of The Expression Of VEGF,bFGF And MVD In Endometrial Carcinoma

Objective:In recent years,with the improvement of the living standards,the incidence of endometrial carcinoma presents a rising trend in the world.Endometrial carcinoma is a set of epitheliogenic malignant tumors which comes from endometrium.The most common is endometrial adenocarcinoma.Endometrial carcinoma is one of the three malignant tumors of female genital tract,which is 7% in female malignant tumors and 20%-30% in female genital tract malignant tumor.The prognosis of endometrial carcinoma is closely related to its surgical pathological stage,histological type,tumor grade,muscular infiltration depth,lymph node metastasis.Therefore,if we can find the biological indicators which is related to the occurrence and development of endometrial carcinoma,and have high sensitivity and specificity,it will provide clinical reference for judging the prognosis of endometrial carcinoma and evaluating therapeutic effect.Vascular endothelial growth factor and basic fibroblast growth factor are Positive regulating angiogenes factors.Microvascular density can react quantitative index of angiogenesis.This research detected the expression of VEGF,bFGF and MVD in normal endometrium tissues and endometrial carcinoma,to explore the effect of VEGF,bFGF and MVD and the relationship among VEGF,bFGF and MVD in occurrence and development of endometrial carcinoma.It will provide new clinical reference for judging the prognosis of endometrial carcinoma and evaluating therapeutic effect,and provide evidence of evidence-based medicine for angiogenesis inhibitors in the treatment of endometrial carcinoma.Methods:1.This study chosed 38 patients who were diagnosed endometrial carcinoma and had received operation 20 patients who had received total hysterectomy because of uterine myoma or ovarian cyst,and whose endometrial pathological results was proliferative endometrium.All patients were from January 2014 to Septemter 2015 in Hebei province general hospital.2.Using immunohistochemical method(SP)technology to detect the expression of VEGF,bFGF and MVD in paraffin section of 38 cases of endometrial carcinoma and 20 cases of normal endometrium.3.Using SPSS17.0 statistical software for statistical analysis,measurement data using t/t 'test,single factor analysis of variance or nonparametric test,count data using test,correlation test uses Spearman rank correlation analysis.Take ?= 0.05 for inspection level,if P is less 0.05,we can think the difference is statistically significant.Results:1.The positive expression rate of VEGF in endometrial carcinoma group was 78.95%,significantly higher than that of normal endometrium group(positive rate is 25%),the difference in two groups was statistically significant(P<0.001).2.The positive expression rate of bFGF in endometrial carcinoma group was 78.95%,significantly higher than that of normal endometrium group(positive rate is 25%),the difference in two groups was statistically significant(P<0.001).3.In endometrial carcinoma group,the expression of MVD is 15.66±1.23/HP,which is significantly higher than that of normal endometrium group(5.6±0.35/HP),he difference in two groups was statistically significant(P<0.001).4.In endometrial carcinoma group,the expression of VEGF and bFGF were positively correlated(r=0.836,P<0.001),the expression of VEGF and MVD were positively correlated(r=0.811,P<0.001),the expression of bFGF and MVD were positively correlated(r=0.782,P<0.001).5.In endometrial carcinoma group,Surgical pathologic stage ?group,the stage ?group,?group,the difference of the positive rate of bFGF in these three groups was not statistically significant(P??=0.440,P??=1.000,P??=0.352);the positive expression rate of bFGF in the muscular wall infiltration depth ?1/2 group was higher than that in the muscular wall infiltration depth <1/2 group,and the difference had statistical significance(P = 0.010);Compared with no lymph node metastasis group,the positive rate of bFGF in with lymph node metastasis group bFGF was higher,but the difference was not statistically significant(P = 0.604).6.In endometrial carcinoma group,Surgical pathologic stage ?group,the stage ?group,?group,the difference of the positive rate of VEGF in these three groups was not statistically significant(P??= 0.689,P?? = 0.273,P??= 0.130);the positive expression rate of VEGF in the muscular wall infiltration depth ?1/2 group was higher than that in the muscular wall infiltration depth <1/2 group,and the difference had statistical significance(P = 0.010);Compared with no lymph node metastasis group,the positive rate of VEGF in lymph node metastasis group VEGF was higher,but the difference was not statistically significant(P = 0.284).7.In endometrial carcinoma group,the expression of MVD in Surgical pathologic stage ?group was highest in these three groups,and the expression of MVD in Surgical pathologic stage group? was higher than the stage ?group,and the difference in these three groups was statistically significant(P??=0.002,P??< 0.001,P??< 0.001);the expression of MVD in the muscular wall infiltration depth ?1/2 group was higher than that in the muscular wall infiltration depth <1/2 group,and the difference had statistical significance(P < 0.001);Compared with no lymph node metastasis group,the expression of MVD in lymph node metastasis group was higher,and the difference was statistically significant(P < 0.001).Conclusions:In endometrial carcinoma,the expression of VEGF,bFGF and MVD increased obviously,and these three factors were positive correlation.MVD is related to surgical pathologic stage,lymph node metastasis and muscular wall infiltration depth,which indicates that local new angiogenesis and rich blood supply plays an important role in the occurrence and development of endometrial carcinoma.VEGF and bFGF is associated with the muscular wall infiltration depth of endometrial carcinoma,but has nothing to do with surgical pathologic stage,and lymph node metastases,which indicates that there are other angiogenic factors except for VEGF,bFGF in the process of the pathological staging and lymph node metastases of endometrial carcinoma,which play a role in the regulation of angiogenesis.