| Objective1.To explore the effects and mechanisms of Guan maining oral liquid on atherosclerosis in Apolipoprotein E gene knockout mice(ApoE-/-mouse),further to define the therapeutic effect of Guan maining oral liquid,thereby providing more powerful experimental evidences.2.To observe the clinical efficacy and safety of Guan maining oral liquid in adjuvant treatment of stable angina pectoris in coronary heart disease with Qi deficiency and blood stasis,and the influence on serum IL-6,hs-CRP,Visfatin.Methods1.Animal experiment adopted ApoE-/-mouse caused by atherosclerosis model in the Guan maining oral liquid intervened,and established the control group,and then observed the levels of serum IL-6,hs-CRP,Visfatin after treatment for 12 weeks.HE-stain was used to observe aortic pathomorphological changes.2.Clinical study selecting 120 cases hospitalized patients with stable angina pectoris in coronary heart disease of cardiovascular department in line with the conditions,were randomly divided into the observation group(60 cases)and control group(60 cases).Conventional western medical therapy was given to both groups.On this basis,the observation group received Guan maining oral liquid.After 3 months we observed the medical indicators of curative effects in both groups and measured the changes of ECG,blood-lipid,the positive ratio of CTA or CAG,clinical hemorheology,levels of serum IL-6,hs-CRP,Visfatin before and after treatment.Results1.Animal experiment:(1)The model group had higher serum levels of TC,TG and LDL-C,as well as high hs-CRP,visfatin and IL-6 expression with Guan maining group and compound salvia miltiorrhiza group(P<0.01 or 0.05).However,the level of HDL-C was lower than the blank control group(P<0.05).(2)Compared with the model group,the serum levels of TC,TG,LDL-C,hs-CRP,Visfatin and IL-6 expression were lower(P all <0.05),while the level of HDL-C was higher in Guan maining group and compound salvia miltiorrhiza group(P<0.05).(3)There was no significant difference between Guan maining group and compound salvia miltiorrhiza group in above-mentioned indicators(P>0.05).(4)The blank control group showed no distinctive changes of atherosclerosis,the model group had distinctive increase of aortic intima thickness and atherosclerotic plaque formation with Guan maining group and compound salvia miltiorrhiza group.Compared with the model group,the area of atherosclerotic lesions and plaques was reduced in Guan maining group and compound salvia miltiorrhiza group.2.Clinical study:(1)The total effective rate of the observation group and control group were 96.7% and 95.0%.There was no significant difference between them(P>0.05).(2)No significant changes was found in ECG features between the two groups(P>0.05).(3)The positive ratio of CTA and CAG of two groups were lowered(P<0.05),and the effect comparison between the two groups showed no significant difference(P>0.05).(4)The levels of TC,TG and LDL-C of both groups were lowered(P all<0.05),and the effect of lowering TC,TG and LDL-C of the observation group was superior to that of control group(P<0.05).(5)The hemodynamic indexes of BV,H?re,L?re,RRI,RAI,Fb,ESR and HCT were improved significantly in both groups(P all < 0.05),and the changes of BV,H?re,L?re,RRI,RAI,Fb,ESR and HCT in the observation group were greater than those of the control group(P all<0.05).(6)The levels of serum IL-6,hs-CRP,Visfatin in both groups after treatment were decreased compared with before this treatment(P all <0.05).Compared with the control group,the levels of serum IL-6,hs-CRP,Visfatin in the observation group after treatment were significantly decreased(P all<0.05).(7)There was no significant difference in routine tests for blood,urine,stool and hepatic and renal function test at pre-and post-treatment(P>0.05).No significant difference was found in safety features between the two groups.Conclusion1.Guan maining oral liquid can increase the activity of ApoE-/-mouse caused by atherosclerosis model.It can inhibit the development of atherosclerosis by lowering serum lipids and the levels of serum IL-6,hs-CRP,visfatin and reducing the plaques area,which is better than salvia miltiorrhiza.2.It is more effective to cure stable angina pectoris in coronary heart disease,and improve myocardial ischemia,decrease the degree of vascular stenosis,reduce blood viscosity,lower serum lipids and the levels of serum IL-6,hs-CRP,visfatin by Guan maining oral liquid with conventional western medical therapy than conventional western medical therapy,and without adverse reaction. |
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