The Experimental Study Of MMP2?MMP9 Expression And Significance During Rats Deep Veinous Thrombosis

Objective: To build successful SD rat model of inferior vena cava thrombosis,during deep vein thrombosis research process. And to investigate MMP-2, the process of the role of the expression of MMP-9 is formed in venous thrombosis.Methods: The weighing about 250-300 g about 60 SD rats were randomly divided into A, B, C, D four groups, A group without treatment, B, C, D group thrombosis model, with a stable thrombus formation modeling the method adopted were anesthetized rats revealed inferior vena cava, the left renal vein and abdominal aorta free, in the lower portion of the renal vein ligation of the inferior vena cava and larger branches. The 1mm diameter guide wire is placed in parallel next to the inferior vena cava, the left renal vein into the inferior vena cava below the ordinary with 3-0 silk suture inferior vena cava ligation together with the guide wire, the guide wire withdrawn. Below the ligature with mosquito clamp clamping the inferior vena cava, the time for about 5 seconds role in mosquito clamp clamp buckle half tooth degrees. 1. 0 ml of normal saline injected into the abdominal cavity, the abdomen was closed. Take A, B, C, D groups vein thrombosis and pathological specimens were used for MMP-2, MMP-9 immunized groups of observation. Fluorescence quantitative PCR were used to detect the expression of mRNA in the process of the inferior vena cava wall of MMP-2, MMP-9 thrombosis.Results: Process modeling experimental group had a certain amount of thrombosis. Modeling process found that there are significant thrombosis 1d, 3d thrombosis arrival, 7d to reduce thrombosis. Immunohistochemistry and quantitative PCR Tip: MMP-2, the expression of MMP-9 in thrombosis significant expression procedure 1d to 3d, when thrombosis 7d expression reduction, group B, group C and group D, statistically different.Conclusion: Ligation rat vena cava vein thrombosis established rat model is relatively simple, high success rate, stable thrombosis, subsequent experiments study the feasibility of controls provided for inferior vena cava thrombosis formation in rats was.MMP-2, MMP-9 in different periods into a process of different trends in the rat vena cava thrombosis expression of deep vein thrombosis is closely some relevance.part 2 MMP2, MMP9 in the rat venous thrombosis is formed during expression...