Study On Hepatoprotective Constituents From Syringa Reticulata

In this paper, the hepatoprotective effects of the ethyl acetate extract of Syringa reticulata were evaluated in D-galactosamine/lipopolysaccharide-induced hepatic failure in mice. The ethyl acetate extract of S. reticulata reduced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, and increased superoxide dismutase (SOD) activity and dicreased malondialdehyde (MDA) content in the liver, The ethyl acetate extract of S. reticulata has a protective effect against the induction of hepatic failure in mice by D-galactosamine and lipopolysaccharide.The bioassay-guided fractionation lead to the isolation of five compounds (1-5) from the ethyl acetate extract of S. reticulate. These compounds were identified as esculetin (1), tyrosol (2),3,4-dihydroxyphenyl ethanol (3), oleuropein (4) syringin (5),8-[2-(3,4-dihydroxy-phenyl)-ethoxycarbonymethyl]-carboxylic acid methyl ester (6) from their spectral data including’H-NMR,13C-NMR,1H-1HCOSY, HMQC and HMBC, (6) is a new compound. Among them, Compounds 1-5 showed significant hepatoprotective effects against tacrine-induced cytotoxicity in Hep G2 cells.