Effect Of Exogenous Leptin On The Proliferation Of Intestinal Mucosa In Rats With Recoverable Obstructive Jaundice

Objective Establish the rats model of recoverable obstructive jaundice,To study the Effect of exogenous leptin on the proliferation of intestinal mucosa in rats with recoverable obstructive jaundice.Methods Establish the rats model of recoverable obstructive jaundice by ligating between the bile duct and the supporting tube,then divide the supporting tube; and Bile duct ligation to establish the model of obstructive jaundice.64 healthy male Wistar rats were randomly divided into 4 groups:ROJ?OJ?ROJ+Leptin?OJ+Leptin. The leptin group were given recombinant leptin by intraperitoneal injection after operation, The other animals were injected with saline at the same time as the control. The rats of the four groups were sacrificed at 5 and 8 days after the surgery, respectively, were labeled as ROJ5, ROJ8, OJ5 and OJ8 group, ROJ5+LP, ROJ8+LP, OJ5+LP and OJ8+LP group. Bleeding to death, laparotomy observation of bile duct obstruction, and specimens. Under the optical microscope section observe the morphological changes of intestinal mucosa structure and measurements of jejunal mucosal villus height and crypt depth; IHC detect the expression of intestinal mucosa leptin, receptor and Ki67 antigen.Results 1. In general, Roj group, OJ group and OJ+LP group respectively at postoperative 4,6,2 days the death 1 only, group ROJ+LP respectively on the 3rd and 5th day after operation the death 1, died because of postoperative intestinal obstruction, intestinal necrosis and peritoneal effusion.2. Intestinal mucosal histological observation:ROJ5 group of intestinal mucosa in order to non-specific inflammatory cell infiltration, interstitial congestion and edema in inflammatory changes and intestinal mucosa atrophy thinning, fuzzy number and density decrease, decreased villus height, increased gap discontinuity, presence of depression and shedding of partial villous, crypt depth becomes shallower, glands sparsely arranged; ROJ8 group of intestinal villus structure is complete, epithelial cells was high columnar, no obvious interstitial edema and neutrophil cells infiltration, and basic maintenance of normal intestinal mucosa structure. The morphology of intestinal mucosa in OJ5 group was similar to that in ROJ5 group, and the morphology of the intestinal mucosa in OJ8 group was much worse than that in OJ5 group, and the infiltration and interstitial congestion of inflammatory cells were common.. The intestinal mucosa damage in ROJ5+LP group was worse than that in ROJ5 group, and there were mild villi disorder, and the morphology of the intestinal mucosa in ROJ8+LP group was not significantly abnormal. The morphology of intestinal mucosa in OJ5+LP group was similar to ROJ5+LP group. The abnormal changes of intestinal mucosal morphology in the OJ8+LP group were decreased, but the degree of the intestinal mucosa morphology was lower than that of the OJ8 group.3. Intestinal mucosal villus height and crypt depth:ROJ5 group of villi height was obviously lower than that of group ROJ8, P< 0.01; OJ5 group of the villus height above OJ8 group, P= 0.05. ROJ5+LP group of villi height significantly lower than in group ROJ8+LP, P< 0.05; OJ5+LP group of the villus height above OJ8+LP group, P< 0.05. ROJ8 group was significantly lower than that of ROJ8+LP group, P< 0.05; OJ8 group of villi height was lower than group OJ8+LP, P< 0.05, ROJ5 group of villi height significantly lower than in group ROJ5+LP, P< 0.01; OJ5 group of villi height was lower than group OJ5+LP, of P< 0.05. The villi in ROJ8 group were significantly higher than that in OJ8 group, P< 0.01, and the villi height of ROJ8+LP group were significantly higher than that of OJ8+LP group, P< 0.01.The crypt depth of ROJ5 group was lower than that of ROJ8 group, the crypt depth of OJ5 group was higher than that of OJ8 group, the crypt depth of ROJ5+LP group was lower than that of ROJ8+LP group, and the crypt depth of OJ5+LP group was higher than that of OJ8+LP group. The crypt depth of intestinal mucosa in ROJ8+LP group was higher than that in ROJ8 group, P< 0.01. ROJ5+LP group of intestinal mucosal crypt depth was significantly higher than that in the group ROJ5, P< 0.01; OJ8+LP group of intestinal mucosal crypt depth was significantly higher than that in the group OJ8, P< 0.01, OJ5+LP group of intestinal mucosal crypt depth significantly higher than group OJ5 and P in the< 0.01. The crypt depth of the ROJ8 group was significantly higher than that of OJ8, P< 0.01, and the villi height of the ROJ+LP group was significantly higher than that of the OJ+LP group, P< 0.05.4. The proliferation of intestinal mucous cells:the positive cells of intestinal mucosa are mainly concentrated in the intestinal mucosal crypt.. ROJ5 group of intestinal mucosal proliferation index was significantly lower than that in the group ROJ8; OJ5 group of intestinal mucosal proliferation index is higher than that of OJ8 group, ROJ5+LP group of intestinal mucosal proliferation index lower than group ROJ8+LP, P< 0.05; OJ5+LP group of intestinal mucosal proliferation index higher than group OJ8+LP. The 8th postoperative day. ROJ8 group of intestinal mucosal proliferation index was significantly lower than that of group ROJ8+LP, P<0.01; OJ8 group of intestinal mucosal proliferation index was significantly lower than that in the group OJ8+LP, P<0.01; OJ5 group of intestinal mucosal proliferation index was significantly lower than that in the group OJ5+LP, P<0.01; group comparison: ROJ8+LP intestinal mucosal proliferation index was significantly higher than that in the group ROJ5+LP, P<0.05, the difference has statistical significance.5. Leptin and leptin in the intestinal mucosa were changed:Leptin and the positive products of the receptor were mainly located in the cytoplasm, and the cytoplasm was brownish yellow particles.. The positive cells were mainly distributed in the intestinal epithelium, crypt cells, intestinal gland cells and lamina cells.. The expression of leptin in the intestinal mucosa of Leptin group and the control group was expressed, but the expression level was different, and the expression was mainly found in the Leptin group, while the expression of the intestinal mucosa in the control group was relatively low or not. The expression of OB in ROJ5 group was significantly higher than that in ROJ8 group, the expression of OB in OJ5 group was higher than that in OJ8 group, and the expression of OB in ROJ5+LP group was lower than that in ROJ8+LP group, and the expression of OB in OJ5+LP group was higher than that in OJ8+LP group. At eighth days after operation, the expression of OB in ROJ8 group was significantly lower than that in ROJ+LP group, P<0.01, and OB expression in OJ8 group was significantly lower than that in OJ8+LP group, P<0.05. At the same time ROJ8+LP group of intestinal mucosa OB protein expression was significantly higher than that of OJ8+LP group, P<0.05. On postoperative day 5. ROJ5 group of intestinal mucosa OB protein expression was significantly lower than that of the ROJ5+LP group, P=0.01; OJ5 group of intestinal mucosa OB protein expression were significantly lower than those in group OJ5+LP, P<0.01.Expression of leptin receptor ROJ5 group was lower than that in group ROJ8; leptin receptor OJ5 group expression group was higher than that of OJ8, leptin receptor ROJ5+LP group expression was lower than that in group ROJ8+LP; leptin receptor OJ5+LP group expression group was higher than that of OJ8+LP.8 days after operation, the leptin receptor ROJ8 group expression was significantly lower than that of the ROJ8+LP group, P<0.01; leptin receptor OJ8 group expression was significantly lower than that of the OJ8+LP group, P<0.05. On postoperative day 5 and leptin receptor ROJ5 group expression was significantly lower than that of the ROJ5+LP group, P<0.01; leptin receptor OJ5 group expression was significantly lower than that of the OJ5+LP group, P< 0.01.Conclusion 1. " ligation between Supporting tube and bile duct plus extubation of the Supporting tube " jaundice model has the advantages of simple operation, relieve jaundice without second laparotomy, small trauma, success rate, the mortality rate is low, is a stable and reliable recovery of obstructive jaundice rats model.2. The obstructive jaundice can decrease the proliferation of intestinal mucosa, which is mainly reflected in the decrease of Ki67, the height of villi and the depth of crypt.. After the lifting of jaundice indicators can be improved.3. Exogenous leptin has the protective effect of promoting the proliferation of intestinal mucosal cells in the obstructive jaundice, and its function can continue to play after the termination of jaundice.. Leptin can accelerate the repair of intestinal mucosal proliferation damage.4. The mechanism of the effect of exogenous leptin on the proliferation of intestinal mucosa cells may be realized by the increase of the expression of leptin in intestinal mucosa and the enhancement of its receptor expression.