Study On The Quality Standard And Fingerprint Of Thlaspi Arvense L.

Thlaspi herba, the dry the ground part of the Thlaspi arvense L. also known as great Shepherd, Ximu, penny cress, Melon seeds, Su baijiang etc, which has the effects of Liver eyesight, treatting fever and ulcers,drainage and detoxification etc, in clinically, it is extensivly used for acute conjunctivitis, abdominal distention and pain, costal pain, intestinal carbuncle, edema, leucorrhea, sore furuncle carbuncle swollen etc.research shows that Thlaspi herba has much pharmacological activities,such as anti-bacterial, anti-viral, anti-inflammatory and painkilling,choleretic and hepatic protective, antipyretic and diuresis etc. In addition,Thlaspi herba, which is also one of the main ingredients of many compound preparations, such as Shisanwei-ximingwan, Huahongpian and Fuyan Jing Jiaonang, and which is widely applied in clinical, and which has obvious effect on easing lots of inflammation and pain.Thlaspi herba are distributed in most parts of China, northern Europe,Africa also distributed, in China it mainly produced in Jiangsu, Zhejiang,Henan and other places. the origin of Thlaspi herba is very widely, the quality of Thlaspi herba which come from different parts is diffrent, many areas in southern of China, the Thlaspi herba, Patrinia scabiosifolia Fisch. Patrinia villosa Juss. and Sonchus oleraceus L. has been mixed to use in Clinically.Comparison with Thlaspi herba, not only in taste, but also owned by thecontained in the active ingredient, the latter three is very different. studies shown that the whole plant of Thlaspi herba main contain black mustard glycosides and other flavonoids such as glycosides and luteolin, isovitexin,apigenin, linarin and diosmetin, which has significant difference with Patrinia scabiosifolia Fisch. Patrinia villosa Juss. and Sonchus oleraceus L. in chemical content.At present, only few paper reported Thlaspi herba's chemical composition and its content determination method, focus on content determination of black mustard glycosides in Thlaspi herba, now, it is rarely related depth study on quality standards of Thlaspi herba. although Thlaspi herba is the new income contained varieties 2010 edition of "Chinese Pharmacopoeia", but the associated quality standards are very scarce, Which is main reasons why confusing mixing using of them in clinically, for Thlaspi arvense L. the current version of "Chinese pharmacopoeia"(2015version) quality standards only provides character identification, qualitative identification and routine checks, and qualitative identification of the reference drug only with reference material, not explain clearly and specific chemical constituents, only use Thlaspi herba medicinal materials as reference for quality evaluation method has many limitation and uncertainty. for more effective control the quality of Thlaspi herba, so in this paper, the quality evaluation methods of Thlaspi herba will be studied systematic.In order to fast analysis and identication of the charateristic composition and achieve effective monitoring of the quality of Thlaspi herba medicine, luteolin and isovitexin was selected as the reference, and thin-layer chromatography was adopted to identifly Thlaspi herba for the first time, established a qualitative method for identification Thlaspi herba medicine; apigenin was selected as the reference, and uv-spectrophometrywith triethylamine-color was used as quality identification for total flavonoids, the hige performance liquied chromatographay was adopted for the content determination of isovitexin, luteolin and acacetin in it.inspected the sample solution and chromatographic conditions(such as mobile phase, detection wavelength, column temperature and flow rate),content determination mothods of Thlaspi herba was established, on this basis, this paper developed draft for quality standards of Thlaspi herba medicine, provides some references for objective evaluation and effective control the quality of Thlaspi herba.At the same time, for comprehensive evaluation and controling the whole quality of Thlaspi herba and it's prescription preparations, in this paper, the fingerprint was carried by the preliminary study of similarity evaluation and comparison of the pattern which come from different habitats, isovitexin was selected as the reference peak to establish fingerprint of 10 batches Thlaspi herba, containing 19 common peaks, in this comon peaks,four components such as isovitexin, luteolin, apigenin and acacia elements was identified, fingerprint analysis similarity evaluation software shows that the 10 batches Thlaspi herba similarity is higer, satisfies the basic demand of similartify evaluation, it can provide a good reference for comprehensive overall asssessment of the quality of Thlaspi herba, it can be provide a scientific basis for Perfecting Thlaspi herba quality evaluation system and achieving effective control the quality and it prescription preparations.